MICROSCOPIC TECHNIQUES LINK TO ABSTRACT OR
FULL TEXT		 PART NO TITLE Journal Volume JISSUE JMONTH JYEAR ABSTRACT SPECIES TISSUE ATCC DESC MORPHOLOGY CELL LINE AUTHOR COMPLETE AUTHORS Company/Institution KEYWORDS MATERIALS & METHODS                                                                                                                                                                                                                                                              
Phase Contrast   890 P35G-0-10-C Pseudomonas-Candida
Interactions: An Ecological Role
for Virulence Factors		 Science 296 5576 June 2002 Bacterial-fungal interactions have great environmental, medical, and economic
importance, yet few have been well characterized at the molecular level. Here,
we describe a pathogenic interaction between Pseudomonas aeruginosa and
Candida albicans, two o		         C. Albicans Deborah A. Hogan Deborah A. Hogan and Roberto Kolter     For microscopy studies, 1 ml of C. albicans was transferred from an overnight culture to a 35-mm Petri dish with a 0.1-mm-thick glass coverslip covering a hole in the bottom of the dish (MatTek Corp., Ashland, MA).                                                                                                                                                                                                                                                              
Phase Contrast Video 781 P35G-1.5-14-C Rho overexpression leads to mitosis-associated detachment of cells from epithelial sheets:
A link to the mechanism of cancer dissemination		 PNAS 101 34 August 2004 Dissemination of neoplastic cells from the primary tumor (invasion and metastasis) is a fundamentally dangerous step in multistage
carcinogenesis. Recent evidence suggests that Rho GTPase-mediated
signaling is linked to dissemination of cells from sever		 Rat Liver   Epithelial IAR-2 Vasilvev, J. M. J. M. Vasiliev*, T. Omelchenko‡§, I. M. Gelfand, H. H. Feder§, and E. M. Bonder Moscow State University, †Oncological Scientific Center of Russia, Rutgers, The State University of New Jersey mitotic spindle metastasis contractility For video microscopy, cells were
grown in glass-bottom chambers (MatTek, Ashland,MA) for 3–5
days.		                                                                                                                                                                                                                                                              
Phase Contrast   638   Measurement of Intracellular Free Zinc Concentrations Accompanying Zinc-Induced Neuronal Death Journal of Neuroscience 19 19 October 1999 Toxic zinc influx may contribute to selective neuronal death after transient global ischemia. We previously used the highaffinity (KD 5 27 nM) fluorescent dye mag-fura-5 to detect initial increases in neuronal intracellular free Zn21 ([Zn 21]i ) associate mouse     neuronal Neocortical Lorella M. Lorella M. T. Canzoniero, Dorothy M. Turetsky, and Dennis W. Choi Washington University voltage-gated calcium channels; calcium; depolarization; kainate; NMDA; neurotoxicity Neurons for intracellular Zn21 imaging experiments were prepared similarly, using 35 mm glass-bottom dishes (MatTek, Ashland, MA) coated with poly-D-lysine/laminin (100:4 ng/ml).                                                                                                                                                                                                                                                              
Phase Contrast Phase Contrast Microscopy 549   Extracellular Cl(-) modulates shrinkage-induced activation of Na(+)/H(+) exchanger in rat mesangial cells. American Journal of Physiology- Cell Physiology 278 6 June 2000 To
examine the effect of hyperosmolality on Na1/H1 exchanger
(NHE) activity in mesangial cells (MCs), we used a pHsensitive
dye, 28,78-bis(2-carboxyethyl)-5(6)-carboxyfluorescein-
AM, to measure intracellular pH (pHi) in a single MC
from rat glomerul		 rat       Mesangial Yukio, Miyata YUKIO MIYATA, SHIGEAKI MUTO, SATORU YANAGIBA, AND YASUSHI ASANO Jichi Medical School intracellular pH; chloride ion; chloride channel; microtubule For pHi measurements, MCs were
plated on 35-mm petri dishes containing a glass coverslip
bottom (MatTec, Ashland, MA) and were used 5–7 days later.
Cells were incubated in 0.5% FBS-containing RPMI 1640 for
24 h before use. Before each experiment, the 		                                                                                                                                                                                                                                                              
Phase Contrast Photomicrographs 400   Potentiation Of Neurite Outgrowth And Reduction Of Apoptosis By Immunosuppressive Agents: Implications For Neuronal Injury And Transplantation Neurosurgical Focus 20 5   2006 Object: Immunosuppressive agents are believed to play a role in recovery from spinal cord injury, but the underlying mechanisms by which neuronal function is improved by these agents are poorly understood. In this study, the authors evaluate the effect of human       SH-SY5Y Sheehan, Jason JASON SHEEHAN, ANNE EISCHEID, RANDI SAUNDERS,
AND NADER POURATIAN		 University of Virginia spinal cord injury, immunosuppressive agent, apoptosis, calcium Undifferentiated SH-SY5Y cells (American Type Culture Collection,
Manassas, VA) at passage numbers between 93 and 100
were plated in 35-mm glass-bottom dishes (MatTek Corp., Ashland,
MA) at densities of between 2 and 4 3 104 cells/dish in high-glucose
DME		                                                                                                                                                                                                                                                              
Phase Contrast   130 35 mm culture dishes CALPAIN ACTIVATION CONTRIBUTES TO DENDRITIC REMODELING AFTER BRIEF EXCITOTOXIC INJURY IN VITRO Journal of Neuroscience 17 3 February 1997 The calcium-dependent protease calpain may contribute to neuronal death in acute neurological insults and may be activated very early in the neuronal injury cascade. We assessed the role of calpain in a model of rapid, reversible dendritic injury in murin           Faddis, B.T. Faddis, B.T., Hasbani, M.J., and Goldberg, M.P.                                                                                                                                                                                                                                                                    
Phase Contrast Phase Contrast Microscopy 250   Membrane-Permeant Chelators Can Attenuate Zn2+-Induced Cortical Neuronal Death Neuropharmacology 45 3   2003 Chelating extracellular Zn2+ with the membrane-impermeant Zn2+ chelator, CaEDTA, can inhibit toxic Zn2+ influx and subsequent neuronal death. However, this drug does not cross the blood–brain barrier. In the present study, we explored the ability of two m       NEURONAL NEURONS Canzoniero, L.Mt. Canzoniero, L.MT., Manzerra, P., Sheline, C.T., and Choi, D.W., Washington University School of  Medicine, St Louis, MO, USA  Neuropharmacology, 45, (), 40-48 (00) Washington University Apoptosis; Neuroprotection; Toxicity; Intracellular zinc Neurons for intracellular Zn2+ imaging experiments were prepared similarly and plated on 35-mm glass-bottom dishes (MatTek, Ashland, MA), coated with poly-D-lysine:laminin (100 ng/ml: 4 ng/ml).                                                                                                                                                                                                                                                              
Phase Contrast Phase-contrast and fluorescence 819 P35G-2-14-CGRD Role of the Midbody Matrix in Cytokinesis: RNAi and Genetic Rescue Analysis of the Mammalian Motor
Protein CHO1		 Molecular Biology of the Cell 15 7 July 2004 CHO1 is a kinesin-like motor protein essential for cytokinesis in mammalian cells. To analyze how CHO1 functions, we
established RNAi and genetic rescue assays. CHO1-depleted cells reached a late stage of cytokinesis but fused back to
form binucleate ce		 Hamster Ovary     CHO Matuliene, Jurgita Jurgita Matuliene and Ryoko Kuriyama University of Minnesota   CHO cells were cultured on photoetched coverslips (Bellco Glass Co., Vineland,
NJ) or in glass-bottom, 3.5-cm culture dishes (MatTek Corp., Ashland,
MA).		                                                                                                                                                                                                                                                              
Phase Contrast Phase Contrast Microscopy 316   Translated Alu Sequence Determines Nuclear Localization Of A Novel Catalytic Subunit Of Casein Kinase 2 American Journal of Physiololgy- Cell Physiology 283 2 August 2002 Casein kinase 2 (CK2) is a tetrameric enzyme constitutively expressed in all eukaryotic tissues. The two known isoforms of the catalytic subunit, CK2 and CK2', have been reported to have distinct tissue-dependent subcellular distributions. We recently des         HuH-7 Hilgard P. Hilgard P., Huang T., Wolkoff, ,  , AW., Stockert,  , RJ. Departments of  Medicine and  Anatomy and Structural Biology and  Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, NY, USA. Am J Physiol Cell Physiology, Albert Einstein College of Medicine nuclear matrix association, human hepatoma cell line HuH-7, casein kinase 2-green fluorescent protein fusion protein ...After 36–40 h, cells were trypsinized and replated on MatTek culture dishes containing a 0.17-mm coverslip in the bottom (MatTek, Ashland, MA)....
...Twenty-four hours after transfection, cells were trypsinized and replated on 35-mm MatTek dishes.…		                                                                                                                                                                                                                                                              
Phase Contrast Phase Contrast Microscopy 576   Expression Of The Tuberous Sclerosis Complex Gene Products, Hamartin And Tuberin, In Central Nervous System Tissues Acta Neuropathologica 99 3 March 2000 Tuberous sclerosis complex (TSC) is a common
genetic disorder in which affected individuals can
develop mental retardation, developmental brain defects,
and seizures. Two genetic loci are responsible for TSC:
TSC1 on chromosome 9q and TSC2 on chromoso		 MOUSE     neuronal C17 Gutmann, David H. David H. Gutmann, Yujing Zhang, M. Josh Hasbani, Mark P. Goldberg, Tracey L. Plank, Elizabeth Petri Henske Washington University Neurons, Astrocytes, Astrocytomas, Glia, Brain Neocortices from day 15 murine embryos were dissociated and
plated on confluent astrocyte cultures at 1 week in vitro, resulting
in mixed astrocyte-neuronal cultures [18]. Preparation of pure neuronal
cultures were generated by plating dissociated neoc