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FULL TEXT		 PART NO TITLE Journal Volume JISSUE JMONTH JYEAR ABSTRACT SPECIES TISSUE ATCC DESC MORPHOLOGY CELL LINE AUTHOR COMPLETE AUTHORS Company/Institution KEYWORDS MATERIALS & METHODS                                                                                                                                                                                                                                                                                                                                                
Flluorescence Epifluorescence 803 P35G-1.5-14-C Phosphorylation of paxillin by p38MAPK is involved in the neurite extension of PC-12 cells Journal of Cell Biology 164 4 February 2004 ell adhesions play an important role in neurite extension. Paxillin, a focal adhesion adaptor protein involved in focal adhesion dynamics, has been
demonstrated to be required for neurite outgrowth. However,
the molecular mechanism by which paxillin reg		   PC-12       Cai Huang Cai Huang,

Christoph H. Borchers,
,
Michael D. Schaller,
,,4
and Ken Jacobson		 University of North Carolina neurons; nerve growth factor; focal adhesions; neurite
outgrowth; mass spectrometry		 Cells stably expressing EGFP-paxillin
or EGFP-paxS85A were plated on collagen-coated MatTek dishes and
treated with 100 ng/ml NGF for 36 h. TIRF and epifluorescence
images were taken as described in Materials and methods. (		                                                                                                                                                                                                                                                                                                                                                
Flluorescence Epifluorescence P50G-1.5-14-F Interleukin-1β affects calcium signaling and in vitro cell migration of astrocyte progenitors Journal of Neuroimmunology     May 2008 Spontaneous calcium activity of neural progenitors is largely dependent on a paracrine signaling mechanism involving release of ATP and
activation of purinergic receptors. Although it is well documented that, in mature astrocytes, cytokines modulate the 		         C57Bl/6 Neurospheres Katharine Striedinger Katharine Striedinger 1, Eliana Scemes   Cytokine; Microglia; Neurospheres; Purinergic receptors; IL-1β; TNFα For in vitro cell
differentiation, floating neurospheres were plated on glass
bottom microwells (MatTek Co, Ashland, MA) coated with
poly-D-lysine-		                                                                                                                                                                                                                                                                                                                                                
Flluorescence Epifluorescence 751 glass bottom 35-mm dishes Force fluctuations and polymerization dynamics of intracellular microtubules PNAS 104 41 October 2007 Microtubules are highly dynamic biopolymer filaments involved in a wide variety of biological processes including cell division,
migration, and intracellular transport. Microtubules are very rigid
and form a stiff structural scaffold that resists deform		 Monkey Kidney     COS-7 Brangwynne, Clifford P. Clifford P. Brangwynne‡, F. C. MacKintosh§, and David A. Weitz Harvard University, Vrije Universiteit cytoskeleton mechanics nonequilibrium persistence length rigidity Cells were sparsely plated onto glass-bottomed 35-mm dishes
(MatTek) and allowed to adhere and spread overnight.		                                                                                                                                                                                                                                                                                                                                                
Flluorescence   852 P35G-1.5-10-C Clustering of endocytic organelles in parental and drug-resistant
myeloid leukaemia cell lines lacking centrosomally organised
microtubule arrays		 International Journal of Biochemistry and Cell Biology       2008 Spatial organisation and trafficking of endocytic organelles in mammalian cells is tightly
regulated and dependent on cytoskeletal networks. The dynamics of endocytic pathways
is modified in a number of diseases, including cancer, and notably in multidr		         HL-60, HL-60/ADR Jing Jin Jing Jin 1, Davide Pastrello, Neal A. Penning, Arwyn T. Jones   Centrosome, Daunorubicin, Doxorubicin, Endosomes
HL-60, Leukaemia, Lysosomes, Microtubules, Multidrug resistance		 Cells were quickly washed with ice-cold
imaging medium (DMEM, 25mM Hepes, pH 7.4, lacking
Phenol Red), resuspended in imaging medium containing
80g/ml unlabelled Tf, and then transferred to 35mm
glass-bottomed dishes (MatTek Corp., Ashland, MA, USA).		                                                                                                                                                                                                                                                                                                                                                
Flluorescence Epifluorescence, TIRF 862 35-mm glass bottom dishes Multiple paxillin binding sites regulate FAK function Journal of Molecular Signaling 3 1 January 2008 Background: FAK localization to focal adhesions is essential for its activation and function.
Localization of FAK is mediated through the C-terminal focal adhesion targeting (FAT) domain.
Recent structural analyses have revealed two paxillin-binding sit		         CHO cells Danielle M Scheswohl Danielle M Scheswohl, Jessica R Harrell, Zenon Rajfur, Guanghua Gao,
Sharon L Campbell and Michael D Schaller		   Paxillin binding sites, FAX Function At 24 hrs,
the transfected cells were plated in 35 mm glass bottom
dishes (MatTek, Ashland, MA) and incubated overnight at
37°C.		                                                                                                                                                                                                                                                                                                                                                
Flluorescence Epifluorescence 755 P35G-1.5-10-C Resolving vesicle fusion from lysis to monitor calcium-triggered lysosomal exocytosis in astrocytes PNAS 104 35 August 2007 Optical imaging of individual vesicle exocytosis is providing new insights into the mechanism and regulation of secretion by cells. To
study calcium-triggered secretion from astrocytes, we used acridine
orange (AO) to label vesicles. Although AO is ofte		 Rat Astrocyte       Jaiswal, Jyoti K. Jyoti K. Jaiswal*, Marina Fix*, Takahiro Takano‡, Maiken Nedergaard‡, and Sanford M. Simon The Rockefeller University, University of Rochester
Medical Center		 acridine orange  imaging  secretion  evanescent wave microscopy  ATP For imaging, cells
were plated onto glass coverslips (Fisher Scientific, Pittsburgh,
PA) or on glass-bottom dishes (MatTek, Ashland, MA) coated
with 1% gelatin (Sigma–Aldrich) and imaged in OptiMEM
(Invitrogen, Carlsbad, CA).		                                                                                                                                                                                                                                                                                                                                                
Flluorescence Epifluorescence 770 35-mm culture dish
that incorporated a #1.5 glass coverslip with a sealed 15-mm
cutout on the bottom		 Compartmentalized signaling of Ras in fission yeast PNAS 103 24 June 2006 Compartment-specific Ras signaling is an emerging paradigm that may explain the multiplex outputs from a single GTPase. The
fission yeast, Schizosaccharomyces pombe, affords a simple system
in which to study Ras signaling because it has a single Ras pro		 Dog Kidney     MDCK Onken, Brian Brian Onken*, Heidi Wiener, Mark R. Philips‡, and Eric C. Chang Baylor College of Medicine, New York University School of Medicine cancer  Cdc42  Int6  mitogen-activated protein kinase  eIF3 To facilitate microscopy (see
Microscopy), cells were imaged in the same 35-mm culture dish
that incorporated a #1.5 glass coverslip with a sealed 15-mm
cutout on the bottom (MatTek, Ashland, MA).		                                                                                                                                                                                                                                                                                                                                                
Flluorescence Epifluorescence, confocal 709 P35G-1.5-14-C Vital imaging of H9c2 myoblasts exposted to tert-butylhydroperoxide-characterization of morphological features of cell death BioMedCentral Cell Biology 8 11 March 2007 Background: When exposed to oxidative conditions, cells suffer not only biochemical alterations,
but also morphologic changes. Oxidative stress is a condition induced by some pro-oxidant
compounds, such as by tert-butylhydroperoxide (tBHP) and can also be		 Rat embryonic heart tissue     H9c2 Sardao, Vilma A Vilma A Sardăo*, Paulo J Oliveira, Jon Holy, Catarina R Oliveira and Kendall B Wallace University of Coimbra, University of Minnesota-Medical School H9c2 myoblasts, H9c2 cell death For epifluorescence or confocal
microscopy, cells were seeded at a density of 35,000
cells per ml in glass-bottom dishes (Mat-Tek Corporation,
Ashland, MA).		                                                                                                                                                                                                                                                                                                                                                
Flourescence Time-Lapse 198   STABLE EXPRESSION OF PROTECTIVE PROTEIN/ CATHEPSIN A-GREEN FLUORESCENT PROTEIN FUSION GENES IN A FIBROBLASTIC CELL LINE FROM A GALACTOSIALIDOSIS PATIENT. MODEL SYSTEM FOR REVEALING THE INTRACELLULAR TRANSPORT OF NORMAL AND MUTATED LYSOSOMAL ENZYMES Biochemical Journal 340 2 June 1999 Fibroblastic cell lines derived from a galactosialidosis patient, stably expressing the chimaeric green fluorescent protein variant (EGFP) gene fused to the wild-type and mutant human lysosomal protective protein/cathepsin A (PPCA) cDNA, were first establ           Naganawa, Y. Naganawa*, Y., Itoh*, K., Shimmoto*, M., Kamei, S., Takiguchi‡, K., Doi‡, H., Sakuraba*, H.