MICROSCOPIC TECHNIQUE LINK TO ABSTRACT OR
FULL TEXT		 PART NO TITLE Journal Volume JISSUE JMONTH JYEAR ABSTRACT SPECIES TISSUE ATCC DESC MORPHOLOGY CELL LINE MAIN AUTHOR Company/Institution KEYWORDS MATERIALS & METHODS                                                          
Confocal   371 P35G-0-14-C Tyrosine Residues Affecting Sodium Stimulation Of Carnitine Transport In The Octn2 Carnitine/Organic Cation Transporter Journal of Biological Chemistry 279 8 February 2004 Primary carnitine deficiency is a disorder of fatty acid oxidation caused by mutations in the Na+-dependent carnitine/organic cation transporter OCTN2. Studies with tyrosyl group-modifying reagents support the involvement of tyrosine residues in Na+ bindi CHINESE HAMSTER OVARY Morphology: Epithelial
Hamster Chinese ovary
Depositor: Dr R Downing, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Nutritional and gene expression studies

Available in the following 		 epithelial-like CHO Amat, Cristina University of Utah primary carnitine deficiency, carnitine transport Genomic DNA was extracted either from fibroblasts or peripheral blood
by standard methods and amplified using PCR and primers flanking
each of the 10 exons of the SLC22A5 gene (4, 5, 17). PCR products were
visualized by agarose gel electrophoresis, pur		                                                          
Confocal   828 P35GC-0-14-C Opposing Roles of Syndecan-1 and Syndecan-2 in Polyethyleneimine-mediated Gene Delivery Journal of Biological Chemistry 283 12 March 2008 Polyethyleneimines (PEIs) are efficient non-viral vectors for gene transfer. Heparan sulfate proteoglycans have been proposed
to be the cell-surface receptors for PEIDNA complexes
(polyplexes). Here, we investigated if syndecan-1 (SDC1) and
syndecan-2		         HEK293-EBNA1 cells Se´bastien Paris Biotechnology Research Institute, National Research Council Canada   SDC1-expressing (a) or SDC2-expressing (b) cells were plated for
1 h on MatTek 3.5-mm Petri dishes. RITC-labeled polyplexes were then added to cells.		                                                          
Confocal   417   Dynamin 2 Is Required For Phagocytosis In Macrophages Journal of Experimental Medicine 190 12 December 1999 Cells internalize soluble ligands through endocytosis and large particles through actin-based phagocytosis.
The dynamin family of GTPases mediates the scission of endocytic vesicles from the
plasma membrane. We report here that dynamin 2, a ubiquitously		         RAW-TT10 Gold, Elizabeth S. University of Washington phagocytosis, macrophages, dynamin, inflammation, phosphoinositide 3-kinase RAW-TT10 cells were transfected with
dyn
K44A
-pTIGZ and plated on 35-mm glass-bottomed microwell
dishes (MatTek Corp.). 18 h later, the dish was mounted on a
Zeiss Axiophot microscope equipped with a cooled CCD camera
(Princeton Instruments) and Me		                                                          
Confocal Laser Scanning 662 MatTek glass bottom
culture dishes (1 mm by 3.5 mm;		 Porphyromonas gingivalis Minor Fimbriae Are Required
for Cell-Cell Interactions		 Infection and Immunity 74 10 October 2006 Two distinctive types of fimbriae have been identified in Porphyromonas gingivalis. In this report, we
demonstrate that minor fimbriae are involved in P. gingivalis autoaggregation and colonization. A mutant with
a deficiency in minor fimbriae can bind to		         Gingival Epithelia Lin, Xinghua Meharry Medical College                                                              
Confocal   409   Aryl Phosphate Derivatives Of Bromo-Methoxy-Azidothymidine Are Dual-Function Spermicides With Potent Anti-Human Immunodeficiency Virus Biology of Reproduction 59 3 September 1998 Detergent-based vaginal microbicides, in addition to their
high contraceptive failure rates, cause mucosal erosion and local
inflammation that might increase the risk of heterosexual human
immunodeficiency virus (HIV) transmission. In a systematic effo		 BOVINE capillary   ENDOTHELIAL HAMSTER EGGS D'Cruz, Osmond J. Wayne Hughes Institute vaginal microbicides, mucosal erosion, inflammation, human
immunodeficiency virus (HIV) transmission, aryl phosphate derivatives		 Frozen hamster eggs (Charles River Laboratories, Wilmington,
MA) were thawed and transferred to 35-mm
glass-bottom microwells (Mat Tek Corporation, Ashland,
MA) in BWW medium. To remove zona, the washed eggs
were treated with 0.05% trypsin (Irvine Sci		                                                          
Confocal   779 P35G-1.5-14-C Nav1.5 E1053K mutation causing Brugada syndrome blocks binding to ankyrin-G and expression of
Nav1.5 on the surface of cardiomyocytes		 PNAS 101 50 December 2004 We identify a human mutation (E1053K) in the ankyrin-binding motif of Nav1.5 that is associated with Brugada syndrome, a fatal
cardiac arrhythmia caused by altered function of Nav1.5. The
E1053K mutation abolishes binding of Nav1.5 to ankyrin-G, and
al		 Rat   Cardiomyocytes     Mohler, Peter J. Duke University Medical Center, University of Pavia, University of Massachusetts Medical School arrhythmia  SCN5A  targeting  trafficking  sudden cardiac death After washes,
buffer was removed and the pellet was resuspended in Vectashield
and plated onto Mattek 35-mm plates.		                                                          
Confocal   841 35-mm glass bottom dishes Heterogeneity and timing of translocation and
membrane-mediated assembly of different annexins		 Experimental Cell Research 314 5 March 2008 Many cell types, including neurons and epithelial cells, express a variety of annexins.
Although the overall function has only been partially unravelled, a dominant feature is the
formation of two-dimensional assemblies under the plasma membrane in a ca		         N1E-115 Tatsiana Skrahina   Annexin, Calcium binding, FRAP, FRET, Translocation For imaging experiments, cells were plated in
35 mm MatTek chambers (Ashland, MA) and transfected with
FuGENE 6 reagent (Roche, Mannheim, Germany) at 50%
confluency.		                                                          
Confocal Time-lapse Confocal 414   Regulated Step In Cholesterol Feedback Localized To Budding Of Scap From Er Membranes Cell 102 3 August 2000 SREBPs exit the ER in a complex with SCAP. Together, they move to the Golgi where SREBP is cleaved, re-leasing a fragment that activates genes encoding lipid biosynthetic enzymes. Sterols block ER exit, pre-venting
cleavage, decreasing transcription, and		         SRD-13A Nohturfft University of Texas SCAP, ER Membranes, Sterols, Cholesterol homeostasis, Sterol Regulatory Element Binding Proteins (SREBPs), cleavage On day 0, cells were set up in glass-bottomed 35 mm dishes (MatTek Co., Ashland, MA) at a density of 3 3 104 cells/dish in medium A supplemented with 5% FCS. On day 2, cells were washed with PBS and refed 1 ml of medium A supplemented with 5% newborn calf                                                          
Confocal   317 P35G-0-10-C The Zinc Transporter Znt3 Interacts With Ap-3 And It Is Preferentially Targeted To A Distinct Synaptic Vesicle  Subpopulation Molecular Biology of the Cell 15 2 February 2004 Synaptic vesicles (SV) are generated by two different mechanisms, one AP-2 dependent and one AP-3 dependent. It has been uncertain, however, whether these mechanisms generate SV that differ in molecular composition. We explored this hypothesis by analyzin RAT ADRENAL Morphology: clusters of small cells
Species: rat; Tissue: adrenal; Tumor: pheochromocytoma
Depositor: Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy
Cell line available for distribution upon consent of the depositor
Properties: the cells are described		 clusters of small cells PC12 Salazar, G. Emory University   Briefly, hippocampi from 1- to 4-day postnatal
rats were enzymatically digested and plated onto either 10-mm #0 MatTek
dishes or 15-mm #0 Deckglaser German glass coverslips coated with Matrigel.		                                                          
Confocal   501   Use Of Confocal Microscopy To Analyze The Rate Of Vancomycin Penetration Through Staphylococcus Aureus Biofilms Antimicrobial Agents and Chemotherapy 49 6 June 2005 When bacteria assume the biofilm mode of growth, they can tolerate levels of antimicrobial agents 10 to 1,000
times higher than the MICs of genetically equivalent planktonic bacteria. The properties of biofilms that give rise
to antibiotic resistance ar		         S Jefferson, Kimberly K. Brigham and Women's Hospital biofilm, Vancomycin
Penetration, Staphylococcus aureus, bacteria		 Biofilms were grown overnight in TSBG in 35-mm collagen-coated
glass coverslip-bottom dishes (MatTek Co., Ashland, MA). The following day,
the media were removed, and the biofilm was washed once in H2O. All confocal
images were obtained at the Harvard 		                                                          
Confocal   795 glass bottom dishes Role of the Regulatory Domain of Protein Kinase D2 in
Phorbol Ester Binding, Catalytic Activity, and
Nucleocytoplasmic Shuttling		 Molecular Biology of the Cell 16 9 September 2005 Protein kinase D2 (PKD2) belongs to the PKD family of serine/threonine kinases that is activated by phorbol esters and
G protein-coupled receptors (GPCRs). Its C-terminal regulatory domain comprises two cysteine-rich domains (C1a/C1b)
followed by a plec		 Human Kidney   Embryonic HEK-293 Auer, Alexandra Medical University of Ulm, Katholieke Universiteit Leuven   For live cell imaging cells were grown and transfected in MatTek glass-bottomed dishes
(MatTek, Ashland, MA).		                                                          
Confocal   694 P35GC-0-14-C culture dishes Neuroendocrine Plasticity in the Anterior Pituitary:  GnRH Mediated Movement in vitro and in vivo Endocrinology 148 4 April 2007 The secretion of luteinizing hormone (LH) is cued by the hypothalamic neuropeptide,
gonadotropin-releasing hormone (GnRH). Following delivery to the anterior pituitary gland via
the hypothalamic-pituitary portal vasculature, GnRH binds to specific high af		 Mice (murine), Ewe Pituitary     T3-1 Navratil University of California San Diego, Colorado State University GnRH Mediated, Anterior Pituitary, Neuroendocrine Plasticity Slices intended for use in video microscopy were individually placed on glass-bottomed
culture dishes (MatTek Corporation) that had been previously coated in poly-D-lysine and
8
collagen (Vitrogen 100, Cohesion).		                                                          
Confocal   695 P35GC-0-14-C Nuclear Autoantigen Translocation and Autoantibody Opsonization Lead to Increased Dendritic Cell Phagocytosis and Presentation of Nuclear Antigens Journal of Immunology 175 4 August 2005 Autoreactivity in lupus requires the delivery of autoantigens to APCs in a proinflammatory context. It has been proposed that
apoptotic cells are a source of lupus autoantigens and targets for autoantibodies. Using a histone H2B-GFP fusion protein as
trac		 Mice bone marrow-derived DCs     K/H2B-GFP Frisoni, Lorenza University of Pennsylvania, The Children’s Hospital of Philadelphia, Temple University School of Medicine Dendritic Cell Phagocytosis, Nuclear Autoantigen Translocation, Autoantibody
Opsonization, Presentation of Nuclear Antigens, Lupus		 K/H2B-GFP cells were cultured in glass-bottom culture
dishes (MatTek). Cells		                                                          
Confocal   405   Interaction Of A Farnesylated Protein With Renal Type Iia Na/Pi Co-Transporter In Response To Parathyroid Hormone And Dietary Phosphate Biochemical Journal 377 3 February 2004 Treatment with PTH (parathyroid hormone) or a high-P(i) diet causes internalization of the type IIa sodium-dependent phosphate (Na/P(i) IIa) co-transporter from the apical membrane and its degradation in the lysosome. A dibasic amino acid motif (KR) in th MONKEY, AFRICAN GREEN KIDNEY IZSBS BS CL103
Morphology: fibroblast-like
Species: monkey, African green; Tissue: kidney; Transformed by: SV40; Original line: CV-1
Properties: virus replication		 fibroblast-like COS-7 Ito, Mikiko Tokushima University School of Medicine endocytosis, internalization, peroxisomal protein,
peroxisomal farnesylated protein (PEX19)		 OK cells were obtained from the American Type Culture
Collection and maintained in an appropriate medium [20]. For
transfection,OKcells were plated in a 35 mmglass-bottomed dish
(MattekCorp.,Ashland, MA,U.S.A.) at a density of 2×105 cells.
Cultures at sub		                                                          
Confocal   643   The SONBNUP98 Nucleoporin Interacts With the
NIMA Kinase in Aspergillus nidulans		 Genetics 165 3 November 2003 The Aspergillus nidulans NIMA kinase is essential for mitotic entry. At restrictive temperature, temperaturesensitive
nimA alleles arrest in G2, before accumulation of NIMA in the nucleus. We performed a screen
for extragenic suppressors of the nimA1 alle		         HeLa De Souza, Colin P. Ohio State University                                                              
Confocal   795 P06G-1.5-20-F Confocal Laser Scanning Microscopy Imaging of the Bovine Corneal Epithelium in vitro Investigative Opthamology & Visual Science       2006 Purpose: Clinical confocal scanning laser microscopes are used in vivo to examine the cornea. However, such instruments are unable to yield the precision and resolution provided by research level in vitro instruments. This study was carried out to evaluat Bovine Cornea   Epithelial   Bantseev, V. University of Waterloo cornea: basic science • cornea: epithelium • mitochondria Following the labeling with fluorescent dyes, the corneal buttons were rinsed twice in HBSS and mounted in 1% agarose, previously dissolved in HBSS, on glass–bottom plates (MatTek Corp., Ashland, MA).                                                          
Confocal   745 P35G-1.5-14-C SNAREpin/Munc18 promotes adhesion and fusion of large vesicles to giant membranes PNAS 105 7 February 2008 Exocytic vesicle fusion requires both the SNARE family of fusion proteins and a closely associated regulatory subunit of the Sec1/
Munc18 (SM) family. In principle, SM proteins could act at an early
SNARE assembly step to promote vesicle–plasma membrane		 Rat       Munc18-1 Tareste, David Columbia University giant liposomes  Sec1/Munc18 Samples
were prepared in glass-bottom dishes (P35G-1.5-14-C; MatTek) coated with 10%
(wt/vol)BSAfor1hatroomtemperature		                                                          
Confocal   441   Loss Of The Homotypic Fusion And Vacuole Protein Sorting Or Golgi-Associated Retrograde Protein Vesicle Tethering Complexes Results In Gentamicin Sensitivity In The Yeast Saccharomyces Cerevisiae Antimicrobial Agents and Chemotherapy 50 2 February 2006 Gentamicin continues to be a primary antibiotic against gram-negative infections. Unfortunately, associated
nephro- and ototoxicity limit its use. Our previous mammalian studies showed that gentamicin is trafficked to
the endoplasmic reticulum in a retr		   kidney     TR Wagner, Mark C. Indiana Center for Biological Microscopy Gentamicin, toxicity, Golgi-associated retrograde
protein (GARP), aminoglycoside, yeast Saccharomyces cerevisiae		 TR was conjugated to gentamicin
as previously described (32). Yeasts were incubated with TR-gentamicin or
lucifer yellow (LY) for the specified period of time under the same growth
conditions as those used for the growth assay. LY accumulates in the ye		                                                          
Confocal   445   Real-Time Optical Monitoring Of Ligand-Mediated Internalization Of A1B-Adrenoceptor With Green Fluorescent Protein Molecular Endocrinology 12 8 August 1998 The study of G protein-coupled receptor signal
transduction and behavior in living cells is technically
difficult because of a lack of useful biological
reagents. We show here that a fully functional a1b-
adrenoceptor tagged with the green fluorescent		 MOUSE       AT3 Awaji, Takeo National Children's Medical Research Center agonist-promoted
a1bAR/GFP redistribution, a1-Adrenoceptors, phospholipase C, Green fluorescent protein		 aT3 cells stably expressing wild-type
a1bAR and a1bAR/GFP were seeded at 1 3 105 per well of the
cover glass-bottom culture dish (MatTek Corp., Ashland, MA)
in 2.0 ml of medium and examined using LSM-GB200 within
30 min at room temperature.		                                                          
Confocal Inverted confocal microscope 588   Mechanisms of Cx43 and Cx26 transport to the plasma membrane and gap junction regeneration Journal of Cell Science 118 19 October 2005 Previous reports have suggested that Cx26 exhibits unique intracellular transport pathways en route to the cell surface compared with other members of the connexin family. To directly examine and compare nascent and steady-state delivery of Cx43 and Cx26          BICR-M1Rk Thomas, Tamsin University of Western Ontario Connexin, Gap junctions, Post-Golgi carriers, Timelapse imaging, Fluorescence recovery after photobleaching BICR-M1Rk cells transiently expressing Cx43-GFP or stably expressing Cx26-YFP were plated in 35 mm glass-bottom tissue culture dishes for live imaging (MatTek Corporation, MA).                                                          
Confocal Laser confocal microscope 315 35-mm
dish (glass bottom, uncoated, no. 0		 Cell Prestress I Stiffness And Prestress Are Closely Associated In Adherent Contractile Cells American Journal of Physiololgy- Cell Physiology 282 3 March 2002 Cell prestress.
I. Stiffness and prestress are closely associated in
adherent contractile cells. Am J Physiol Cell Physiol 282:
C606–C616, 2002. First published October 31, 2001; 10.1152/
ajpcell.00269.2001.—The tensegrity hypothesis holds that
the c		 human MUSCLE     HASM Wang, N. Harvard School of Public Health tensegrity, mechanical stress, traction, cytoskeleton, actin microfilaments First, about five drops of 0.1 N NaOH were added to the 35-mm dish (glass bottom, uncoated, no. 0; MatTek, Ashland, MA) and air-dried.                                                          
Confocal   626 P35, 1cm Nuclear Localization of the 82-kDa Form of Human Choline Acetyltransferase Journal of Biological Chemistry 274 27 July 1999 Choline acetyltransferase is the enzyme catalyzing synthesis of the neurotransmitter acetylcholine in cholinergic neurons. In human, transcripts encoding two forms of the enzyme with apparent molecular masses of 69 and 82 kDa are found in brain and spinal         HEK-293 Resendes, Manuel C. University of Western Ontario Choline acetyltransferase, enzyme catalyzing synthesis, brain, spinal cord, amino acid, cell nucleus For confocal microscopy, cells were replated at 24 h after transfection onto 35-mm dishes containing a 1-cm glass-bottomed center well (Plastek Cultureware, Ashland, MA) in growth medium supplemented with 20 mM HEPES buffer, pH 7.4.                                                          
Confocal   821 P35GC-1.5-10-C Simvastatin inhibits Staphylococcus aureus host cell invasion through modulation of isoprenoid intermediates Journal of Pharmacology And Experimental Therapeutics 326 1 July 2008 Patients on a statin regimen are at a decreased risk of death due to bacterial sepsis. We have found that protection by simvastatin includes the inhibition of host cell invasion
by Staphylococcus aureus, the most common etiologic agent of sepsis. Inhibit		 Human Umbilical Cord   Endothelial HUVEC Horn, Mary P. Ball State University, Indiana School of Medicine   For confocal imaging,
cells were plated as above on 35 mm glass-bottom dishes (MatTek, Ashland, MA)
coated as above.		                                                          
Confocal   793 P06G-1.5-20-F Distribution of Organelles Identified in Epithelium of Rat Corneas in vivo Using Confocal Laser Scanning Microscopy Investigative Opthamology and Visual Science 48     2007 Purpose:The purpose of this study was to investigate the feasibility of using confocal laser scanning microscopy together with specific fluorescent markers to assess the distribution of cellular organelles in corneal epithelium of rat in vivo.

Methods		 Rat Cornea   Epithelial   Bantseev, V. University of Waterloo, Bausch&Lomb, Inc. cornea: basic science • mitochondria • imaging/image analysis: non-clinical Animals (n=5) were then placed on a custom-designed heated microscope stage (to maintain body temperature) with the cornea facing glass-bottom multi-well plates (MatTek Corp., Ashland, MA).                                                          
Confocal   224   Dynamic Analysis Of Stat6 Signalling In Living Cells FEBS Letters 532 1 December 2002 Functional activity of N- and C-terminal fluorescent fusion proteins between STAT6 and EGFP was demonstrated through IL-4-dependent transcriptional activation and nuclear translocation. The N-terminal (EGFP–STAT6) fusion protein appeared to be more active HUMAN KIDNEY, EMBRYONIC DSMZ ACC 305
Morphology: adherent fibroblastoid cells growing as monolayer
human embryonal kidney established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5); classified as risk category 1 according to the German Central Comm		 epithelial HEK-293 Nelsona, G. University of Liverpool STAT6; NF-kappaB; Confocal microscopy; Green fluorescent protein For confocal microscopy, cells were plated onto 35-mm Mattek dishes (Mattek, USA) at 1.6×105 cells in 2 ml of medium. HeLa cells (ECACC No. 93021013) were grown in MEM with Earle’s salts, plus 10% FCS, and 1% NEAA at 37°C, 5% CO2….                                                          
Confocal   577   Kinetics Of Core Histones In Living Human Cells: Little Exchange Of H3 And H4 And Some Rapid Exchange Of H2B Journal of Cell Biology 153 7 June 2001 Histones H2A and H2B form part of the
same nucleosomal structure as H3 and H4. Stable HeLa
cell lines expressing histones H2B, H3, and H4 tagged
with green fluorescent protein (GFP) were established;
the tagged molecules were assembled into nucleosome		 HUMAN cervix IZSBS BS TCL20
Morphology: epithelial-like
Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid
Properties: antitumour testing; transformation; tumorigenicity; cytotoxicity; cell biology; bacterial invasiveness; virology		 epithelial-like HeLa Kimura, Hiroshi University of Oxford cell fusion, FRAP, histone actetylation, nucleosome, transcription General procedures for fixation, immunolabeling, and image collection
using an MRC1000 confocal microscope have been described (Kimura et
al., 1999; Manders et al., 1999). Cells grown on glass-bottomed dishes
(Mat-Tek) in 100 ng/ml Hoechst 33342 (Harag