MICROSCOPIC TECHNIQUES	Atomic Force Microscopy  (AFM)	Atomic Force Microscopy  (AFM)	Atomic Force Microscopy  (AFM)	Atomic Force Microscopy  (AFM)	Atomic Force Microscopy  (AFM)	Atomic Force Microscopy  (AFM)
LINK TO ABSTRACT OR FULL TEXT	426	492	357	489	589	483
MATTEK PART NO CITED			P35G-1.5-14-C		P35G-1.5-10-C
TITLE	Novel Dynamic Rheological Behavior Of Individual Focal Adhesions Measured Within Single Cells Using Electromagnetic Pulling Cytometry	Atomic Force-Multi-Optical Imaging Integrated Microscope For Monitoring Molecular Dynamics In Live Cells	Mechanism Of Prion Propagation: Amyloid Growth Occurs By Monomer Addition Amyloid Growth Occurs By Monomer Addition	Elasticity Of Native And Cross-Linked Polyelectrolyte Multilayer Films	Atomic Force Microscopy Study of Early Morphological Changes during Apoptosis	Surface Probe Measurements Of The Elasticity Of Sectioned Tissue, Thin Gels And Polyelectrolyte Multilayer Films:Correlations Between Substrate Stiffness And Cell Adhesion
Journal	Acta Biomaterialia	Journal of Biomedical Optics	PLoS Biology	Biomacromolecules	Langmuir	Surface Science
Volume	1	10	2	5	21	570
JISSUE	3	6	9	5	20	1-2
JMONTH	May	November/December	September	September	September	October
JYEAR	2005	2005	2004	2004	2005	2004
ABSTRACT	The rheology of cells and sub-cellular structures, such as focal adhesions, are important for cell form and function. Here we describe electromagnetic pulling cytometry (EPC), a technique to analyze cell rheology by applying dynamic tensional forces to	A novel hybrid imaging system is constructed integrating atomic force microscopy  AFM  with a combination of optical imaging techniques that offer high spatial resolution. The main application of this instrument  the NanoFluor microscope is the study	Abundant nonfibrillar oligomeric intermediates are a common feature of amyloid formation, and these oligomers, rather than the final fibers, have been suggested to be the toxic species in some amyloid diseases. Whether such oligomers are critical intermed	Mechanical properties of polyelectrolyte multilayer films were studied by nanoindentation using the atomic force microscope (AFM). Force-distance measurements using colloidal probe tips were systematically obtained for supported films of poly(L-lysine)	Apoptosis is defined by a distinct set of morphological changes observed during cell death including loss of focal adhesions, the formation of cell membrane buds or blebs, and a decrease in total cell volume. Recent studies suggest that these dramatic mor	Surface probe measurements of the elasticity of thin film matrices as well as biological samples prove generally important to understanding cell attachment across such systems. To illustrate this, sectioned arteries were probed by atomic force microscop
SPECIES	BOVINE	HUMAN		RAT
TISSUE		AORTA		aorta thoracic, embryonic		MUSCLE
ATCC DESC				Morphology: fibroblast-like Species: rat, DB1X; Tissue: aorta thoracic, embryonic Properties: biochemistry; biophysics Available in the following LABORATORY:
MORPHOLOGY				fibroblast-like
CELL LINE	ALVEOLAR TYPE II	VSMCs	Coleoptiles victoriae from oat	A7R5	KB	A7R5
AUTHOR	Overby, Darryl R.	Trache	Collins, Sean R.	Richert, Ludovic	Hessler, Jessica A.	Engler, Adam J.
COMPLETE AUTHORS	Darryl R. Overby, Benjamin D. Matthews, Eben Alsberg, Donald E. Ingber	Andreea Trache, Gerald A. Meininger	Sean R. Collins, Adam Douglass, Ronald D. Vale, Jonathan S. Weissman	Ludovic Richert, Adam J. Engler, Dennis E. Discher, and Catherine Picart	Jessica A. Hessler, Andrew Budor, Krishna Putchakayala, Almut Mecke, Daniel Rieger, Mark M. Banaszak Holl, Bradford G. Orr, Anna Bielinska, James Beals, and James Baker, Jr.	Adam J. Engler, Ludovic Richert, Joyce Y. Wong, Catherine Picart, Dennis E. Discher
Company/Institution	Children's Hospital Boston	Texas A&M University	HHMI	Universite Louis Pasteur	University of Michigan	University of Pennsylvania
KEYWORDS	Cell mechanics; Cytoskeleton; Focal adhesion; Creep; Magnetic	integrated microscopy; atomic force microscopy; total internal reflection fluorescence; Forster resonance energy transfer; internal reflection microscopy	prion inheritance, polymerization, NM, a fragment of the Sup35 protein containing the glutamine/asparagine-rich N-terminal (N) and highly charged middle (M) domains, amyloid formation	polyelectrolyte multilayer films, Biopolymers, cell adhesion	apoptosis, apoptotic volume decrease (AVD), Atomic force microscopy (AFM)	Surface structure, morphology, roughness, and topography; Adhesion; Atomic force microscopy
MATERIALS & METHODS	Bovine capillary endothelial cells were cultured in 10% CO2 at 37  C on gelatin-coated tissue culture dishes in Dulbeccos modified Eagles medium (DMEM, Gibco-BRL) supplemented with 10% calf serum (CS; Hyclone), 10 mM Hepes (JRH-Biosciences), 2 mM L	The VSMCs were isolated from a rat cremaster arteriole as previously described.68 Low passage VSMCs were trypsinized and then centrifuged to form a pellet. The cell pellet was dispersed in cell culture media, and the cells were cultured on glass-botto	For continuous thioflavin T assay measurements,  concentrated stocks of NM stored in either 6 M GuHCl (fluorescently labeled NM and NM used for Figures 2C, 2F, and 6) or 4 M GuHCl and 4 M urea (NM used for all other experiments) were diluted at least 2	A7r5 smooth muscle cells (SMC) were maintained in polystyrene flasks between passages 2 and 15, and cultured in media containing DMEM, supplemented with 10% of fetal bovine serum and antibiotics. Cells were passed every 3 days, when reaching 80% conf	KB cells were plated 1 day in advance on MatTek tissue culture dishes (glass bottom No. 15, uncoated, ç-irradiated, Part # P35G-1.5-10-C) in RPMI 1640 media.	A7r5 SMCs (aorta-derived cell line) were maintained in polystyrene flasks between passages 2 and 15, and cultured in media containing DMEM, supplemented with 10% of fetal bovine serum and antibiotics. Cells were passed every 3 days, when reaching 80