MICROSCOPIC TECHNIQUES HYPERLINK to PAPER or ABSTRACT MIC CATEGORY PART NO TITLE JOURNAL VOLUME ISSUE MONTH YEAR ABSTRACT SPECIES TISSUE ATCC DESC MORPHOLOGY CELL LINE MAIN AUTHOR KEYWORDS MATERIALS & METHODS
Video 599 Video-rate confocal   Fluorogenic Phospholipids as Head Group-Selective Reporters of Phospholipase A Activity ACS Chemical Biology 1 2 March 2006 PLA (phospholipases A) are important mediators of cell signaling, generating bioactive fatty acids and LPLs (lysophospholipids). PLA products having different head groups can initiate vastly different types of signaling. Fluorogenic analogues of the PLs ( mouse     neuronal SCG neurons Rose, Tyler M. phospholipases A, cell signaling, lisophospholipids, PLA2 enzymes Dissociated neurons from 8–16 week-old SCG were plated at a density of one-half ganglion per one 35 mm poly-l-lysine-coated glass bottom petri dish (no. 1.5, MatTek Corp.) and incubated at 37 °C in a 5% CO2 environment in MEM.
Video 632 Electron Microscopy   Arp23 complex-deficient mouse fibroblasts are viable and have normal leading-edge actin structure and function PNAS 102 45 November 2005 RNA interference silencing of up to 90% of Arp3 protein expression, a major subunit of the Arp2 3 complex, proportionately decreases the intracellular motility of Listeria monocytogenes and actin nucleation activity ascribable to the Arp2 3 complex in mou mouse       embryonic fibroblasts Di Nardo, Alessia RNA interference, motility, ruffling Cells were seeded on 35 mm glass-bottom dishes (MatTek, Ashland, MA) and microinjected with 1 pg of CFP-CA or CFP-VCA vector DNA by using a MO-150 micromanipulator and an IM200 pressure injector (Narishige, Tokyo).
Video 661 Microscopic Observation   Growth Cone Collapse and Inhibition of Neurite Growth by
Botulinum Neurotoxin C1: A t-SNARE Is Involved in Axonal Growth		 The Journal of Cell Biology 134 1 July 1996 Abstract. The growth cone is responsible for axonal
growth, where membrane expansion is most likely to
occur. Several recent reports have suggested that presynaptic
proteins are involved in this process; however,
the molecular mechanism details are unclea		 chick dorsal root     ganglion Igarashi, Michihiro   Video-enhanced Microscopic Observation
We cultured E7 chick DRG explants on the laminin-coated glass-bottom
culture dish (MatTek Corp., Ashland, MA). A video microscopy analysis
using video-enhanced contrast-differential interference contrast (VECDIC)
was
Video 818 Time-Lapse P35GC-1.0-14-C Cohesin Is Dispensable for Centromere Cohesion in Human Cells PLoS ONE 2 3 March 2007 Proper regulation of the cohesion at the centromeres of human chromosomes is essential for accurate genome transmission. Exactly how cohesion is maintained and is then dissolved in anaphase is not understood. Principal Findings.
We have investigated the 		         HeLa Diaz-Martinez, Laura A.   HeLa cells were plated onto poly-d-lysine
coated 35 mm tissue culture dishes fitted with glass cover-slips
(MatTek Cultureware).
Video 199     TRANSLOCATION OF RNA GRANULES IN LIVING NEURONS Journal of Neuroscience 16 24 December 1996 Sorting of RNAs to specific subcellular loci occurs in diverse settings from fly oocytes to mammalian neurons. Using the membrane-permeable nucleic acid stain SYTO 14, we directly visualized the translocation of endogenous RNA in living cells. Labeled RNA           Knowles, R.    
Video 552 Video Enhanced Contrast Differential Interference Contrast (VEC-DIC)   Suicide Gene Therapy Using Adenovirus Vector For Human Oral Squamous Carcinoma Cell Line In Vitro Nagoya Journal of Medical Science 67 3-4   2005 Recently, suicide gene therapy using the herpes simplex virus thymidine kinase (HSVtk) gene followed
by ganciclovir (GCV) administration was evaluated for the treatment of cancer. The purpose of this study
was to investigate the effectiveness of suicide		 HUMAN KIDNEY, EMBRYONIC DSMZ ACC 305
Morphology: adherent fibroblastoid cells growing as monolayer
human embryonal kidney established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5); classified as risk category 1 according to the German Central Comm		 epithelial HEK-293 Yamamoto, Noriyuki Squamous Cell Carcinoma, Adenovirus, Herpes Simplex Virus Thymidine Kinase Gene,
Ganciclovir, Bystander effect		 We plated 2×104 cells/ml of the SAS line into a glass bottomed culture dish (Mat Tec
Corporation, USA) and incubated a 5% CO2-incubator at 37°C for 24 hours. Then the medium
was changed, the cells were infected with AxCAHSVtk vectors at an MOI of 10, an
Video 669   35 mm glass bottom microwell dishes Characterization of Interactions of Adapter Protein RAPL/Nore1B with RAP GTPases and Their Role in T Cell Migration Journal of Biological Chemistry 282 42 October 2007 Using a model of integrin-triggered random migration of T
cells, we show that stimulation of LFA-1 integrins leads to the
activation of Rap1 and Rap2 small GTPases. We further show
that Rap1 and Rap2 have distinct roles in adhesion and random
migration of		 Human chimeric protein     ICAM-1Fc Miertzschke T Cell Migration, Adapter Protein
RAPL/Nore1B, RAP GTPases		 Briefly, 35-mm glass bottom microwell dishes
(MatTek Corp., Ashland, MA) were
coated at 4 °C overnight with 3
g/ml ICAM-1Fc in phosphatebuffered
saline and then blocked
with 2.5% bovine serum albumin in
phosphate-buffered saline
Video 536 Time-Lapse   Partial Cell Fusion: A Newly Recognized Type Of Communication Between Dedifferentiating Cardiomyocytes And Fibroblasts Cardiovascular Research 68 1 October 2005 Fibroblasts have been shown to couple to neonatal cardiomyocytes in heterocellular cultures through functional gap junctions.
Our objective was to provide evidence for an additional type of heterocellular communication between fibroblasts and adult cardi		         HEK-293 Driesen. Ronald B. Cell communication; Cardiomyocytes; Fibroblasts; Gap junctions; Partial cell fusion; Annexin V New Zealand white rabbits (ca. 2 kg) were used for this
study. Cardiomyocytes and cardiac fibroblasts were isolated from adult rabbits by a retrograde collagenase perfusion as
previously described [11]. In brief, rabbits were anaesthetized
by intra-art
Video 457 Video microscopy, immunofluorescence microscopy   Golgi Duplication In Trypanosoma Brucei Journal of Cell Biology 165 3 May 2004 Duplication of the single Golgi apparatus in the
protozoan parasite
Trypanosoma brucei
has been
followed by tagging a putative Golgi enzyme and a
matrix protein with variants of GFP. Video microscopy
shows that the new Golgi appears de novo, near to		   THYMUS   brucei T. He, Cynthia Y. Golgi apparatus, Duplication,Trypanosoma brucei, de novo biogenesis T. brucei cells were plated on the surface of agarose gels prepared in cultivation
medium and air dried briefly to limit cell motility. The gel was
placed in an imaging dish (MatTek) with the cell side facing the coverslip.
Cells were imaged at 28C in
Video 484 Digital Videomicroscopy   Astrocytes Regulate Developmental Changes In The Chloride Ion Gradient Of Embryonic Rat Ventral Spinal Cord Neurons In Culture Journal of Physiology 509 3 June 1998 Embryonic rat ventral spinal cord neurons were dissociated at day 15 and grown on: (i) polyd-
lysine (PDL); (ii) a confluent monolayer of type I astrocytes; or (iii) PDL in astrocyteconditioned
medium (ACM) to examine the influence of astroglia on the r		 RAT SPINAL CORD   NEURONAL   Li, Yong-Xin chloride ion gradient, GABAergic potential, amino acids, glial fibrillary acid protein
(GFAP),		 Pregnant Sprague—Dawley rats were narcotized by COµ
inhalation followed by cervical dislocation. E15 embryos were
removed from the uteri by Caesarean section, rapidly decapitated
and placed in phosphate-buffered saline at room temperature
(20—22 °C). 
Video 351 Video Enhanced Contrast Differential Interference Contrast (VEC-DIC) P35G-0-14-C Process Of Apoptosis Induced By Tnf-A In Murine Fibroblast Ltk-Cells: Continuous Observation With Video Enhanced Contrast Microscopy Apoptosis 7 1 February 2002 Apoptosis Is originally defined by unique morphological changes of dying cells, and the biochemical hallmark associated with apoptosis is internucleosomal DNA fragmentation. However, few report has shown the precise time course of the apoptotic events. Th         Murine K. Okamoto apoptosis, caspase, Dnase ?, Ltk-, TNF-a, VEC-DIC microscopy The murine fibroblast cell line Ltk-, which is highly sensitive to TNF-a, was maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum, 0.1 mM nonessential amino acids, 5 mM
L-glutamine, and antibiotics (streptomycin, 
Video 196 Scanning electron microscopy   MARCKS REGULATES MEMBRANE RUFFLING AND CELL SPREADING Current Biology 7 8 August 1997 The dynamic rearrangement of the actin cytoskeleton is fundamental to most biological processes including embryogenesis, morphogenesis, cell movement, wound healing and metastasis. Membrane ruffling and reversible cell–substratum interactions underlie act           Myat, M.    
Video 893   glass bottom dishes The conformational state of Tes regulates its
zyxin-dependent recruitment to focal adhesions		 Journal of Cell Biology 161 1 April 2003 he function of the human Tes protein, which has
extensive similarity to zyxin in both sequence and
domain organization, is currently unknown. We now
show that Tes is a component of focal adhesions that, when
expressed, negatively regulates proliferati		         T47D Boyan K. Garvalo Tes; focal adhesion; zyxin; RNAi; conformation 10 5 viable T47D cells/ml (wild-type or stably expressing GFP-Tes or GFP)
were plated onto fibronectin (10

g/ml) coated MatTek dishes.
Video 233 Time-Lapse   Analysis Of Dna Fragmentation Of In Vitro Cultured Bovine Blastocysts Using Tunel Theriogenology 57 9 June 2002 Programmed cell death (apoptosis) characteristically affects the single cells of blastocysts whereas necrosis affects cluster of cells in both the inner cell mass (ICM) and the trophectoderm (TE). This study uses the trophectodermrminal deoxynucleotidyl t BOVINE EMBRYO       Neuber, E. Apoptosis; Blastocysts; Bovine; Parthenogenotes; TUNEL Prior to the live observations the parthenogenotes and the fertilized embryos were placed in a glass-bottom chamber (MatTek Corp., Ashland, MA) filled with culture medium and overlaid with pre-warmed mineral oil.
Video 604   P35GC Ionized Intracellular Calcium Concentration Predicts Excitotoxic Neuronal Death: Observations with Low-Affinity Fluorescent Calcium Indicators Journal of Neuroscience 17 17 September 1997 Cytosolic calcium ([Ca21]i ) is an important mediator of neuronal signal transduction, participating in diverse biochemical reactions that elicit changes in synaptic efficacy, metabolic rate, and gene transcription. Excessive [Ca21]i also has been implica mouse     neoronal Neocortical Hyrc, Krzysztof AMPA, calcium, excitotoxicity, fura-2, glutamate, kainate, NMDA, videomicroscopy Cells were plated (0.5 hemisphere per dish) onto glass-bottom 35 mm dishes (Mattek, Ashland, MA) previously coated with a mixture of poly-D-lysine (0.5 mg/ml) and laminin (4 mg/ml).
Video 463     Viewing Cell Movements In The Developing Neuroendocrine Brain Integrative and Comparative Biology 43 6 December 2003 Many studies suggest that migratory guidance cues within the developing brain are diverse
across many regions. To better understand the early development and differentiation of select brain regions,
an in vitro method was developed using selected inbred		 rat  basophilic leukemia   neuronal HEADS Tobet, Stuart A. brain development, GFP, ventromedial nucleus of the hypothalamus Heads or brains are dissected free from mouse embryos
in cold Krebs buffer. After embedding in 8%
low melting temperature agarose (Sigma Type VII-A),
slices (250 mm thick) were cut using a vibrating microtome
(Leica VT1000S) and placed into cold steri