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FULL TEXT		 PART NO TITLE Journal Volume JISSUE JMONTH JYEAR ABSTRACT SPECIES TISSUE ATCC DESC MORPHOLOGY CELL LINE AUTHOR COMPLETE AUTHORS Company/Institution KEYWORDS MATERIALS & METHODS
Time-Lapse Time-lapsed video microscopy, laser scanning Confocal 563   Replication Of Cryptococcus Neoformans In Macrophages Is Accompanied By Phagosomal Permeabilization And Accumulation Of Vesiclescontaining Polysaccharide In The Cytoplasm PNAS 99 5 March 2002 Cryptococcus neoformans (CN), an encapsulated, ubiquitous environmental
yeast, is pathogenic for humans, primarily those with
compromised immune function. CN is believed to be a facultative
intracellular pathogen. Time-lapsed video microscopy revealed		 MOUSE BALB/c; Tumor: sarcoma, histiocytic cell Morphology: stellate
Species: mouse, BALB/c; Tumor: sarcoma, histiocytic cell
Properties: antigen presentation; phagocytosis; chemotaxis; cytotoxicity		 STELLATE J774 Tucker, Stephanie C. Stephanie C. Tucker and Arturo Casadevall Albert Einstein College of Medicine replication, phagosomal
permeabilization, Cryptococcus neoformans, phagosome		 Infected or treated cells [where infections or treatments were performed on glass coverslips in MaTek (Ashland, MA)-brand
dishes] were washed with PBS to remove trace serummedium.
Monolayers were fixed with 3.7% formaldehyde in 1Fix Buffer
pH 7.2 (
Time-Lapse time-lapse fluorescence microscopy, immunofluorescence microscopy 555   Baf Is Required For Emerin Assembly Into The Reforming Nuclear Envelope Journal of Cell Science 114 24 December 2001 Mutations in emerin cause the X-linked recessive form of
Emery-Dreifuss muscular dystrophy (EDMD). Emerin
localizes at the inner membrane of the nuclear envelope
(NE) during interphase, and diffuses into the ER when
the NE disassembles during mitosis.		 HUMAN cervix IZSBS BS TCL20
Morphology: epithelial-like
Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid
Properties: antitumour testing; transformation; tumorigenicity; cytotoxicity; cell biology; bacterial invasiveness; virology		 epithelial-like HeLa Haraguchi, Tokuko Tokuko Haraguchi, Takako Koujin, Miriam Segura-Totten, Kenneth K. Lee, Yosuke Matsuoka,
Yoshihiro Yoneda, Katherine L. Wilson and Yasushi Hiraoka		 Japan Science and Technology Corporation Nuclear envelope, Lamin A, Lamin-associated
polypeptide 2, MAN1, Emery-Dreifuss muscular dystrophy, Barrierto-
autointegration factor, Chromosome		 Cells were grown in a glass-bottom culture dish (MatTech, USA).
GFP fusion plasmids (1 mg) were transfected into cells with
LipofectaminePlus (Gibco BRL) according to manufacturer’s
methods except that the incubation time with DNA was reduced to
1.5 h
Time-Lapse time-lapse fluorescence microscopy 425   Identification Of Novel Principles Of Keratin Filament Network Turnover In Living Cells Molecular Biology of the Cell 15 5 May 2004 It is generally assumed that turnover of the keratin filament system occurs by exchange of subunits along its entire length
throughout the cytoplasm. We now present evidence that a circumscribed submembranous compartment is actually the
main site for ne		         eukaryotic Windoffer, Reinhard Reinhard Windoffer, Stefan Wo¨ ll, Pavel Strnad, and Rudolf E. Leube Johannes Gutenberg University keratin, intermediate filaments, Keratin filaments For time-lapse fluorescence microscopy of living cells, phenol-red–free
Hanks’ medium (Invitrogen GmbH) was used. Images were recorded by
epifluorescence microscopy using inverse optics (Olympus, Hamburg, Germany)
and an attached IMAGO slow-scan CCD ca
Time-Lapse   479   Sindbis Virus-Induced Neuronal Death Is Both Necrotic And Apoptotic And Is Ameliorated By N-Methyl-D-Aspartate Receptor Antagonists Journal of Virology 75 15 August 2001 Virus infection of neurons leads to different outcomes ranging from latent and noncytolytic infection to cell
death. Viruses kill neurons directly by inducing either apoptosis or necrosis or indirectly as a result of the host
immune response. Sindbis vi		   HIPPOCAMPAL   NEURONAL Primary Nargir-Aizenman, Jennifer L. JENNIFER L. NARGI-AIZENMAN AND DIANE E. GRIFFIN Johns Hopkins University Neuronal Death, apoptosis,  necrosis, Sindbis virus Primary cortical cells were prepared from gestational
day 18 Long-Evans rats as previously described (56). Briefly, the cortex
was dissected in Hanks balanced salt solution and digested in 10 U of papain
(Worthington Biochemical, Lakewood, N.J.)/ml in 
Time-Lapse Time-lapse imaging; FRAP 225   Mobile Foci Of Sp100 Do Not Contain Pml: Pml Bodies Are Immobile But Pml And Sp100 Proteins Are Not Journal of Structural Biology 140 1-3 October 2002 PML bodies are nuclear organelles that are associated with various diseases and are suggested to be involved in multiple cellular activities including transcriptional regulation, apoptosis, and antiviral defence. Because many proteins with different funct HUMAN BONE Morphology: epithelial-like
Species: human, Caucasian female 15 years old; Tissue: bone; Tumor: osteosarcoma; Validated by isoenzymes: PGM1,1; PGM3,2; ES D,1; AK1,1; GLO-1,2; G6PD,B
Properties: antitumour testing; cytotoxicity		   U2OS Wiesmeijer, K. Wiesmeijer, K.,  Molenaar, C., Bekeer, I., Tanke H., and Dirks R., Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands. Journal of Structural Biology, 40, (-), 80-88, (00) Leiden University PML; Sp100; CBP; Time-lapse imaging; FRAP "VH10 human primary fibroblasts and U2OS human osteosarcoma cells were cultured on 3.5-cm glass bottom petri dishes (Mattek, Ashland, MA) …"
Time-Lapse time-lapse microscopy 593   A Preformed Complex of Postsynaptic Proteins Is Involved in Excitatory Synapse Development Neuron 49 4 February 2006 Nonsynaptic clusters of postsynaptic proteins have been documented; however, their role remains elusive. We monitored the trafficking of several candidate proteins implicated in synaptogenesis, when nonsynaptic clusters of scaffold proteins are most abund rat     neuronal HIPPOCAMPAL Gerrow, Kimberly Kimberly Gerrow, Stefano Romorini, Shahin M. Nabi, Michael A. Colicos, Carlo Sala, and Alaa El-Husseini University of British Columbia postsynaptic proteins, synaptogenesis, scaffolding proteins, neuroligin-1, postsynaptic density (PSD) Neurons were plated at 100,000 per 35mm glass-bottom microwell dish (MatTek).
Time-Lapse Time-lapse video phase-contrast microscopy and time-lapse digital confocal-fluorescence microscopy 341   Macropinocytosis Is The Endocytic Pathway That Mediates Macrophage Foam Cell Formation With Native Low Density Lipoprotein Journal of Biological Chemistry 280 3 January 2005 Previously, we reported that fluid-phase endocytosis
of native LDL by PMA-activated human monocytederived
macrophages converted these macrophages
into cholesterol-enriched foam cells (Kruth, H. S.,
Huang, W., Ishii, I., and Zhang, W. Y. (2002) J. Biol		 HUMAN FORESKIN     HFF Kruth, H. Kruth, H., Jones, N., Huang, W., Zhao, B., Ishii, I., Chang, J., Combs, C., Malide, D., and Zhang, W-Y. Section of Experimental Atherosclerosis, NHLBI, National Institutes of Health, Bethesda, MD., the Department of Pathology, Wake Forest Unive National Institutes of Health    
Time-Lapse Time-Lapse Video Microscopy 474   Dynamic Interactions Of Macrophages With T Cells During Antigen Presentation Journal of Experimental Medicine 190 12 December 1999 We have established a method for real-time video analysis of the interaction of antigen-presenting
cells (APCs) with T cells. Green fluorescent protein expression controlled by a nuclear factor of
activated T cells (NFAT)-responsive promoter permits the		 MOUSE monocyte macrophage ECACC 91062702
Morphology: Macrophage
Mouse monocyte macrophage
Depositor: Not registered
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Immunoglobulin, complement Products: Lysozyme Applications: Metabolic studies		   RAW 264.7 Underhill, David M. David M. Underhill,
Michael Bassetti,
Alexander Rudensky,
and Alan Aderem		 University of Washington nuclear factor of activated T cells, hybridoma, green fluorescent protein,
video microscopy		 RAW 264.7 cells were plated
48 h before use on 35-mm glass-bottomed microwell dishes
(MatTek Corp.) with 20 U/ml IFNg
, and OVA was added to
10 mg/ml for the final 16 h. DO11-GFP cells were added to the
dish at fivefold excess, centrifuged onto the R
Time-Lapse Time-lapse microscopy 475   A Pseudomonas Aeruginosa Quorum-Sensing Molecule Influences Candida Albicans Morphology Molecular Microbiology 54 5 December 2004 Candida albicans
is an opportunistic pathogen that is
commonly found as a member of the human microflora.
The ability of
C. albicans
to alter its cellular
morphology has been associated with its virulence;
yeast cells are more prevalent in commensa		 YEAST       CANDIDA ALBICAN Hogan, Deborah A. Deborah A. Hogan,
Åshild Vik and Roberto Kolter		 Harvard Medical School Candida albicans, 3OC12HSL, fungal
morphology, pathogen, Pseudomonas aeruginosa		 Candida albicans was grown in 5 ml of YNBNP medium incubated
in a rollerdrum overnight at 378C. The morphological
state of C. albicans was determined microscopically using a
100¥ objective lens on a Nikon Eclipse TE300 inverted microscope
equipped wit
Time-Lapse Time-lapse movies 318   A Critical Step In Metastasis: In Vivo Analysis Of Intravasation At The Primary Tumor Cancer Research 60 9 May 2000 Detailed evaluation of all steps in tumor cell metastasis is critical for evaluating the cell mechanisms controlling metastasis. Using green fluorescent protein transfectants of metastatic (MTLn3) and nonmetastatic (MTC) cell lines derived from the rat ma         MTLn3-GFP Wyckoff, Jb. Wyckoff, JB., Jones, JG., Condeelis, , JS., Segall, JE.  Department of Anatomy and Structural Biology,   Pathology, and  the Analytical Imaging Facility Albert Einstein College of Medicine, Bronx, NY, USA. Cancer Research, 60, (9), 504-5, (00 Albert Einstein College of Medicine   The lungs were then placed in matTek dishes (MatTek Corporation, Ashland, MA) with 1 ml of L15 medium (Life Technologies, Inc.) to keep them moist.
Time-Lapse   733 35-mm poly-d-lysine-coated glass bottom dishes Spatiotemporal control of spindle midzone formation by PRC1 in human cells PNAS 106 16 April 2006 We have examined the role of PRC1, a midzone-associated, microtubule
bundling, Cdk substrate protein, in regulating the spatiotemporal
formation of the midzone in HeLa cells. Cdk-mediated
phosphorylation of PRC1 in early mitosis holds PRC1 in an inactive
         HeLa Zhu, Changjun Changjun Zhu, Eric Lau, Robert Schwarzenbacher, Ella Bossy-Wetzel, and Wei Jiang Burnham Institute for Medical Research Cdk phosphorylation  mitosiscytokinesis  microtubule-associated
proteins  microtubule bundling		 HeLa cells grown on a 35-mm polylysinecoated
glass-bottom microwell dish (MatTek, Ashland, MA) were
transfected with mammalian expression plasmids andor siRNA by
using Lipofectamine 2000 or Oligofectamine (Invitrogen).
Time-Lapse Time-lapse microscopy 500   Retinal Axon Growth Cones Respond To Ephb Extracellular Domains Asinhibitory Axon Guidance Cues Development 128 15 August 2001 Axon pathfinding relies on cellular signaling mediated by
growth cone receptor proteins responding to ligands, or
guidance cues, in the environment. Eph proteins are a
family of receptor tyrosine kinases that govern axon
pathway development, including		         E14 Birgbauer, Eric Eric Birgbauer, Stephen F. Oster, Christophe G. Severin and David W. Sretavan University of Californa, San Francisco (UCSF) Axon pathfinding, Ephrins, Extracellular domains,
Retinal axons, Reverse signaling, Mouse		 E14 dorsal retinal explants were grown overnight in 35 mm
coverslip culture dishes (MatTek Corporation). Cultures were
overlaid with prewarmed mineral oil (Sigma or Fisher) and
equilibrated on a 37°C microscope stage incubator with CO2 influx.
Glass m
Time-Lapse   884 35-mm glass bottom dishes mDia2 induces the actin scaffold for the contractile ring and stabilizes its position
during cytokinesis in NIH 3T3 cells		 Molecular Biology of the Cell 19 5 May 2008 mDia proteins are mammalian homologs of Drosophila diaphanous and belong to the
formin family proteins that catalyze actin nucleation and polymerization. While formin
family proteins of nonmammalian species such as Drospohila diaphanous are essential in		         NIH 3T3 Sadanori Watanabe Sadanori Watanabe, Yoshikazu Ando, Shingo Yasuda, Hiroshi Hosoya, Naoki
Watanabe, Toshimasa Ishizaki and Shuh Narumiya		   mDia2, Actin Scaffold, Contractile Ring, NIH 3T3, Cytokinesis NIH 3T3 cells were seeded on 35-mm glass-bottom dishes (Mat Tek) with or without
siRNA transfection.
Time-Lapse   886 glass bottom dishes Calpain-mediated regulation of the distinct signaling pathways
and cell migration in human neutrophils		 Journal of Leukocyte Biology 84   July 2008 We studied the mechanisms underlying
calpain inhibition-mediated human neutrophil migration.
MAPKs, including ERK, p38, and JNK,
MEK1/2, MAPK kinase 3/6 (MKK3/6), PI-3K/Akt,
c-Raf, and p21-activated kinase (PAK; an effector
molecule of Rac) were rapi		         HL-60 Masataka Katsube Masataka Katsube, Takayuki Kato, Maki Kitagawa, Haruyoshi Noma, Hisakazu Fujita,
and Seiichi Kitagawa		   Mitogen-activated protein kinases,  pertussis toxin phosphatidylinositol 3-kinase, Rac Cells (3.5105/ml) suspended in HBSS were placed in a glass-bottom dish
(MatTek Co., Ashland, MA, USA)
Time-Lapse   912 P50G-1.5-3x14-F Cell and fibronectin dynamics during branching
morphogenesis		 Journal of Cell Science 119 16 August 2006 Branching morphogenesis is a dynamic developmental
process shared by many organs, but the mechanisms that
reorganize cells during branching morphogenesis are not
well understood. We hypothesized that extensive cell
rearrangements are involved, and inv		         E12 and E13 SMGs Melinda Larsen Melinda Larsen, Cindy Wei and Kenneth M. Yamada   Branching morphogenesis, Migration, Salivary gland,
Fibronectin		 Whole SMGs were cultured as previously described (Hoffman et al., 2002)
except that for imaging they were cultured submerged in glass-bottom culture
dishes (MatTek) coated with FN.
Time-Lapse   761 glass bottom culture dishes Microtubule function in fibroblast spreading is modulated according to the tension state
of cell–matrix interactions		 PNAS 104 13 March 2007 Mechanical and physical features of the extracellular environment dramatically impact cell shape. Fibroblasts interacting with 3D
relaxed collagen matrices appear much different from cells on 2D
collagen-coated surfaces and form dendritic cell extension		 Human foreskin fibroblasts       Rhee, Sangmyung Sangmyung Rhee, Hongmei Jiang, Chin-Han Ho, and Frederick Grinnell University of Texas Southwestern Medical Center adhesion  cell plasticity  cytoskeleton  extracellular matrix
mechanosignaling		 Time-lapse microscopy
was carried out with glass bottom culture dishes (MatTek,
Ashland, MA) placed in a 37°C environment chamber.
Time-Lapse time-lapse microscopy 418   Imaging Movement Of Malaria Parasites During Transmission By Anopheles Mosquitoes Cellular Microbiology 6 7 July 2004 Malaria is contracted when
Plasmodium
sporozoites
are inoculated into the vertebrate host during the
blood meal of a mosquito. In infected mosquitoes,
sporozoites are present in large numbers in the secretory
cavities of the salivary glands at the m		         SALIVARY Frischknecht, Friedrich Friedrich Frischknecht,
Patricia Baldacci,
Béatrice Martin,
Christophe Zimmer,
Sabine Thiberge,
Jean-Christophe Olivo-Marin,
Spencer L. Shorte
and Robert Ménard		 Institut Pasteur Malaria, mosquito, Plasmodium
sporozoites		 The Plasmodium berghei NK65 strain transformed to express
GFP at the sporozoite stage was described previously (Natarajan
et al., 2001). Parasite stocks were refreshed after several months
by natural transmission from mosquitoes to mice. Mice developin
Time-Lapse Time-Lapse Videomicroscopy 578   Apoptotic Membrane Blebbing Is Regulated By Myosin Light Chain Phosphorylation Journal of Cell Biology 140 3 February 1998 The evolutionarily conserved execution
phase of apoptosis is defined by characteristic changes
occurring during the final stages of death; specifically
cell shrinkage, dynamic membrane blebbing, condensation
of chromatin, and DNA fragmentation. Mechan		 RAT ADRENAL MEDULLA     PC12 Mills, Jason C. Jason C. Mills, Nicole L. Stone, Joseph Erhardt, and Randall N. Pittman University of Pennsylvania membrane blebbing, apoptosis, z-VAD-FMK, myosin light chain kinase inhibitors, cell contraction, execution phase For Hoechst staining after serum removal or staurosporine treatment,
300,000 cells were plated on collagen-coated, glass bottom 35-mm tissue
culture dishes (Mat-Tek, Ashland, MA). After experimental treatments,
cells were rinsed three times with PBS
Time-Lapse three-dimensional time-lapse microscopy 430   Cdc25B cooperates with Cdc25A to induce mitosis
but has a unique role in activating cyclin B1–Cdk1
at the centrosome		 Journal of Cell Biology 171 1 October 2005 Cdc25 phosphatases are essential for the activation
of mitotic cyclin–Cdks, but the precise roles of the
three mammalian isoforms (A, B, and C) are unclear.
Using RNA interference to reduce the expression of
each Cdc25 isoform in HeLa and HEK293 cells		 HUMAN cervix IZSBS BS TCL20
Morphology: epithelial-like
Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid
Properties: antitumour testing; transformation; tumorigenicity; cytotoxicity; cell biology; bacterial invasiveness; virology		 epithelial-like HeLa Lindqvist, Arne Arne Lindqvist, Helena Källström, Andreas Lundgren, Emad Barsoum, and Christina Karlsson Rosenthal Karolinska Institutet mitosis, cyclin B1–Cdk1, phosphorylation Cells growing on a glass-bottomed dish (MatTek) or a coverslip were microinjected
with 10 nM siRNA and 0.008

g/

l plasmid CFP (pCFP)-
Golgi as a marker in the first release of a double thymidine block. As for
time-lapse microscopy, different si
Time-Lapse time-lapse microscopy, fluorescent stereomicroscopy 453   Using Green Fluorescent Malaria Parasites To Screen For Permissive Vector Mosquitoes Malaria Journal 5 23 March 2006 The Plasmodium species that infect rodents, particularly Plasmodium berghei and
Plasmodium yoelii, are useful to investigate host-parasite interactions. The mosquito species that act
as vectors of human plasmodia in South East Asia, Africa and South Ame		 zebrafish     NEUROEPITHELIA MOSQUITOES Frischknecht Friedrich Frischknecht, Beatrice Martin, Isabelle Thiery,
Catherine Bourgouin and Robert Menard		 Institut Pasteur malaria, sporozoites, Plasmodium berghei Whole mosquitoes or isolated midguts were observed at
10–18 days (A. stephensi and A. gambiae) or 10–26 days
(A. albimanus) after the infectious blood meal. Sporozoites
were isolated from infected salivary glands 15–22
days (A. stephensi) and 15–35 da
Time-Lapse Time-lapse Microscopy, Indirect Immunofluorescence 236   Role Of Pi 3-Kinase And Pip3 In Submandibular Gland Branching Morphogenesis, Developmental Biology Developmental Biology 255 1 March 2003 The mouse submandibular gland (SMG) epithelium undergoes extensive morphogenetic branching during embryonic development as the first step in the establishment of its glandular structure. However, the specific signaling pathways required for SMG branching          E13 Larsen, M. Larsen, M., Hoffman, M.P., Sakai, T., Neibaur, J.C., Mitchell, J.M, and Yamada, K.M., Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, USA. Develop National Institutes of Health  Branching morphogenesis; Salivary gland; Submandibular gland; Organ culture; Phosphatidylinositol 3-kinase; Phosphatidylinositol 3,4,5-trisphosphate; Akt; Inhibitors; Mouse; Time-lapse microscopy The SMG were cultured on membranes at the air/medium interface at 37°C for 1–72 h. Five or more glands were placed on Nuclepore Track-Etch Membranes (Whatman, Clifton, NJ) (13 mm, 0.1 µM) floating on 250 µL of DMFTV standard media [DMEM/F12 (1:1) (Invitro
Time-Lapse Time-lapse video microscopy 435   Lfa-1/Icam-1 Interaction Lowers The Threshold Of B Cell Activation By Facilitating B Cell Adhesion And Synapse Formation Immunity 20 5 May 2004 The
integrin LFA-1 and its ligand ICAM-1 mediateB cell adhesion, but their role in membrane-bound antigen recognition
is still unknown. Here, using planar lipid bilayers
and cells expressing ICAM-1 fused to green fluorescence protein, we found that the		   LYMPHOCYTE     adipocyte Carrasco, Yolanda R. Yolanda R. Carrasco, Sebastian J. Fleire,
Thomas Cameron, Michael L. Dustin,
and Facundo D. Batista		 Lincoln's Inn Fields Laboratories Cell Adhesion, synapse, B cell receptor (BCR) Time-lapse video microscopy was performed using a Nikon Diaphot
200 inverted microscope equipped with a CCD camera (Hama-matsu Orca). ICAM-1-GFP APCs were split on 35 mm glass-bottom dishes (MatTek Corporation, Ashland, MA). The dish was placed on a 37 C
Time-Lapse   676 P35G-1.5-14-C A Surfactant Protein C Precursor Protein BRICHOS Domain Mutation Causes Endoplasmic Reticulum Stress…. American Journal of Respiratory Cell and Molecular Biology 32 6 June 2005 BRICHOS is a domain found in several proteins consisting of  100
amino acids with sequence and structural similarities. Mutations in
BRICHOS domain have been associated with both degenerative and
proliferative diseases in several nonpulmonary organs, alt		 Human embryonic kidney     HEK 293 Mulugeta, Surafel Surafel Mulugeta, Vu Nguyen, Scott J. Russo, Madesh Muniswamy, and Michael F. Beers University of Pennsylvania School of Medicine conformational disease; apoptosis; unfolded protein response;
epithelial cells; protein aggregation		 For time-lapse microscopy, the same procedure
described above was followed with the exception of using glass-bottom
35-mm petri dishes with 0.16- to 0.19-mm coverglasses (MatTek Corporation,
Ashland,MA) for live microscopic observation.
Time-Lapse Time-lapse videomicroscopy 532   Contrasting Effects Of Basic Fibroblast Growth Factor And Neurotrophin 3 On Cell Cycle Kinetics Of Mouse Cortical Stem Cells Journal of Neuroscience 22 15 August 2002 Basic fibroblast growth factor (bFGF) exerts a mitogenic effect
on cortical neuroblasts, whereas neurotrophin 3 (NT3) promotes
differentiation in these cells. Here we provide evidence
that both the mitogenic effect of bFGF and the differentiationpromot		 MOUSE CORTICAL     STEM CELLS Lukaszewicz Agnes Lukaszewicz, Pierre Savatier, Ve´ ronique Cortay, Henry Kennedy, and Colette Dehay Institut National de la Sante et de la Recherche Medicale G1 phase; cell cycle; proliferation; neuroblast;
time-lapse videomicroscopy; bFGF; NT3; corticogenesis		 Cortical precursors from green fluorescent protein (GFP; Okabe et al.,
1997) / and / mice were seeded on poly-L-lysine- and laminin-coated glass coverslips to obtain 25% of GFP cells. This low proportion
of GFP cells makes it possible to reliably
Time-Lapse Time-lapse video microscopy, Scanning Electron Microscopy, Immunofluorescence, Digital Immunofluorescence Microscopy 337   Early Molecular Events In The Assembly Of The Focal Adhesion-Stress Fiber Complex During Fibroblast Spreading Cell Motility and the Cytoskeleton 58 3 July 2004 Cell adhesion to the extracellular matrix triggers the formation of integrin-mediated contact and reorganization of the actin cytoskeleton. Examination of nascent adhesions, formed during early stages of fibroblast spreading, reveals a variety of forms of human foreskin   FIBROBLAST FOAM Zimerman, B. Zimerman, B., Volberg, T., and Geiger, B. Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel. Cell Motility and the Cytoskeleton 58, (), 4-59, (004). The Weizmann Institute of Science   After the electroporation,
the cells were collected by centrifugation, resuspended in
DMEM, and plated on glass-bottom microwell dishes
(MatTek, Ashlend, MA)
Time-Lapse   702 P35G-1.5-14-C Shape Change Controls Supporting Cell Proliferation in Lesioned Mammalian Balance Epithelium Journal of Neuroscience 27 16 April 2007 Mature mammals are uniquely vulnerable to permanent auditory and vestibular deficits, because the cell proliferation that produces
replacement hair cells in other vertebrates is limited in mammals. To investigate the cellular mechanisms responsible for th		 Mouse Embryonic utricles     Embryonic urticles Meyers, Jason R. Jason R. Meyers and Jeffrey T. Corwin University of Virginia hair cell; regeneration; lysophosphatidic acid; LPA; vestibular; wound healing; cytoskeleton Embryonic utricles were adhered with
the nerve side down on poly-L-lysine-coated glass-bottom dishes (Mat-
Tek, Ashland, MA). Utricles from postnatal animals were adhered nerve
side down in MatTek dishes treated with CellTak (2l, air-dried onto the
glass
Time-Lapse   641   Rac1 null mouse embryonic fibroblasts are motile and respond to PDGF Molecular Biology of the Cell 17 5 May 2006 Previous studies of Rac1 in fibroblasts have used dominant negative constructs, which
may have non-specific effects. We used a conditional Rac1 allele to critically examine Rac1
function in mouse fibroblasts. Lack of Rac1 had dramatic effects on non-confl		         MEF Vidali, Luis Luis Vidali*, Feng Chen, Gregor Cicchetti*, Yasutaka Ohta*, and David J. Kwiatkowski*‡ Brigham and Women’s Hospital and Harvard Medical School,   Mouse alleles, PCR and Southern blot Rac1 conditional and null alleles were generated as previously reported (Glogauer et al.,
2003; Gu et al., 2003). The conditional Rac1 allele contains loxP sites flanking exon1; upon cremediated
excision this allele ge
Time-Lapse   814 P35G-1.5-14-C The retromer complex and clathrin define an early endosomal retrograde exit site Journal of Cell Science 120 12 June 2007 clathrin adaptors AP1 and epsinR, and the retromer complex in retrograde sorting from early/recycling endosomes to the trans Golgi network (TGN). However, it
has remained unclear whether these protein machineries
function on the same or parallel pathway		         HeLa Popoff, Vincent Vincent Popoff, Gonzalo A. Mardones, Danièle Tenza, Raúl Rojas, Christophe Lamaze,
Juan S. Bonifacino, Graça Raposo and Ludger Johannes		 The retromer complex and clathrin define an early
endosomal retrograde exit site		 Retrograde transport, Retromer, Shiga toxin, Endosome,
Golgi, Clathrin		 HeLa cells grown to 40-50% confluency on 35-mmglass-
bottom culture dishes (MatTek Corporation,
Ashland, MA)
Time-Lapse   713 glass-bottom microwell dishes p120 catenin regulates lamellipodial dynamics and cell adhesion in cooperation with cortactin PNAS 104 26 June 2007 The armadillo-family protein, p120 catenin (p120), binds to the
juxtamembrane domain of classical cadherins and increases cell–
cell junction stability. Overexpression of p120 modulates the activity
of Rho family GTPases and augments cell migratory abilit		 Mouse cDNA in pRcCMV vector (46)     p120 Boguslavsky, Shlomit Shlomit Boguslavsky*, Inna Grosheva*, Elad Landau*, Michael Shtutman, Miriam Cohen*, Katya Arnold*,
Elena Feinstein‡, Benjamin Geiger*, and Alexander Bershadsky*§		 The Weizmann Institute of Science, Ordway Research Institute,
Inc., QBI Enterprises Ltd.,		 adherens junctions  Arp2/3  endocytic vesicles  focal adhesions
leading edge		 For live cell imaging, cells were plated on glass-bottom microwell
dishes (MatTek, Ashland, MA). Movies were taken using a
DeltaVision microscopy system (Applied Precision, Issaquah,
WA).
Time-Lapse time-lapse laser scanning microscopy 482   Effect Of Tumor-Associated Mutant E-Cadherin Variants With Defects In Exons 8 Or 9 On Matrix Metalloproteinase 3 Journal of Cellular Physiology 202 3 August 2004 Tumor progression is characterized by loss of cell adhesion and increase of invasion and metastasis. The cell adhesion molecule
E-cadherin is frequently down-regulated or mutated in tumors. In addition to down-regulation of cell adhesion, degradation of 		 HUMAN BREAST    Epithelial MDA-MB-435S Fuchs, Margit MARGIT FUCHS, CHRISTINE HERMANNSTA¨ DTER, KATJA SPECHT, PJOTR KNYAZEV,
AXEL ULLRICH, ERIKA ROSIVATZ, RAYMONDE BUSCH, PETER HUTZLER,
HEINZ HO¨ FLER,
AND BIRGIT LUBER		 Technische Universitat Munchen Tumor progression, cell adhesion, matrix metalloproteinase 3, E-cadherin, cell motility For time-lapse laser scanning microscopy, cells were seeded
at a density of 2105 cells per 3.5-cm in collagen I-coated
plates with a glass bottom which were purchased from MatTek
Corporation (Ashland, MA). Uncoated plates were coated for
4hat 378Cwit
Time-Lapse   731 35-mm glass bottom dishes Different mitochondrial intermembrane space proteins are released during apoptosis in a manner
that is coordinately initiated but can vary in duration		 PNAS 103 31 August 2006 The release of mitochondrial intermembrane space proteins to the
cytosol is a key event during apoptosis. We used in situ fluorescent
labeling of proteins tagged with a short tetracysteine-containing
sequence to follow the release of Smac, Omi, adenylate 		         HeLa Munoz-Pinedo, Cristina Cristina Mun˜ oz-Pinedo*, Ana Guı´o-Carrio´ n‡§, Joshua C. Goldstein‡, Patrick Fitzgerald*, Donald D. Newmeyer‡,
and Douglas R. Green		 St. Jude Children’s Research Hospital, Institut de Recerca Oncolo` gica–Institut d’Investigacio´ Biome` dica de Bellvitge (IDIBELL), ‡La Jolla Institute for Allergy and Immunology apoptosis-inducing factor  cytochrome c  mitochondria  Omi  Smac For time-lapse analysis, cells were grown in poly-D-lysine coated 35-mm glass-bottom microwell dishes (MatTek), and treated in 3 ml of DMEM (GIBCO) supplemented with 10% FCS/20 mM Hepes, pH 7.3/2 mM L-glutamine/200 mg/ml penicillin/100 mg/ml streptomycin 
Time-Lapse   697 35 mm glass bottom culture
dishes		 Proteolysis restricts localiztion of CID, the centromere-specific histone H3 variant of Drosophila, to centromeres Nucleic Acids Research 34 21 December 2006 Centromere identity is determined by the formation
of a specialized chromatin structure containing the
centromere-specific histone H3 variant CENP-A.
The precise molecular mechanism(s) accounting
for the specific deposition of CENP-A at centromeres
are st		   D.melanogaster     Kc167 Moreno-Moreno, Olga Olga Moreno-Moreno, Monica Torras-Llort and Fernando Azorın Parc Cientıfic de Barcelona   For in vivo time-lapse analysis, 1.2 · 106 cells were plated
48 h post-transfection on 35 mm glass-bottomed culture
dishes (Mattek Inc.),
Time-Lapse time-lapse microscopy, Confocal 530   Mitochondrial Metabolism Reveals A Functional Architecture In Intact Islets Of Langerhans From Normal And Diabetic Psammomys Obesus American Journal of Physiology- Endocrinology and Metabolism 287 6 December 2004 The cells within the intact islet of
Langerhans function as a metabolic syncytium, secreting insulin in a
coordinated and oscillatory manner in response to external fuel. With
increased glucose, the oscillatory amplitude is enhanced, leading to
the hy		         Islet Katzman, S. M. S. M. Katzman, M. A. Messerli, D. T. Barry, A. Grossman, T. Harel,
J. D. Wikstrom, B. E. Corkey, P. J. S. Smith, and O. S. Shirihai		 Tufts University diabetes; metabolic oscillations; mitochondrial membrane potential;
rhodamine 123		 Mitochondrial membrane potential
was measured using the potentiometric dye Rh123 (Molecular
Probes, Eugene, OR) loaded at a final concentration of 13 M. This
concentration of dye is not harmful to the cells; in studies of isolated
-cells, exposure o
Time-Lapse   261   Distinct Functions Of The Unique C Terminus Of Lap2 A In Cell Proliferation And Nuclear Assembly Journal of Biological Chemistry 277 27 May 2002 The non-membrane-bound lamina-associated polypeptide 2 isoform, LAP2, forms nucleoskeletal structures with A-type lamins and interacts with chromosomes in a cell cycle-dependent manner. LAP2 contains a LEM (LAP2, emerin, and MAN1) domain in the constant N HUMAN cervix IZSBS BS TCL20
Morphology: epithelial-like
Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid
Properties: antitumour testing; transformation; tumorigenicity; cytotoxicity; cell biology; bacterial invasiveness; virology		 epithelial-like HeLa Vlcek, S. Vlcek, S., Barbara Korbei ,  B., and Foisner, R., Department of Biochemistry and Molecular Cell Biology, Vienna Biocenter, University of Vienna, Vienna, Austria. Journal of Biol. Chem., 77, (), 8898-8907, (00). University of Vienna   For time lapse microscopy, cells were seeded on glass-bottom culture dishes (MatTek Corp., Ashland, MA), grown for 8-48 h after transfection, and incubated …
Time-Lapse   382   Macrophages Promote The Invasion Of Breast Carcinoma Cells Via A Colony-Stimulating Factor-1/Epidermal Growth Factor Paracrine Loop Cancer Research 65 12 June 2005 Previous studies have shown that macrophages and tumor cells are comigratory in mammary tumors and that these cell types are mutually dependent for invasion. Here we show that macrophages and tumor cells are necessary and sufficient for comigration and in   Breast     MTLN3-GFP Goswami, Sumanta Sumanta Goswami, Erik Sahai, Jeffrey B. Wyckoff, Michael Cammer, Dianne Cox,
Fiona J. Pixley, E. Richard Stanley, Jeffrey E. Segall, and John S. Condeelis		 Albert Einstein College of Medicine Colony-Stimulating Factor-1/Epidermal
Growth Factor, chemotactic factor		 MTLn3-GFP and RAW264.7 (LR5) cells were cultured in
a-MEM with 5% fetal bovine serum (FBS). BAC1.2F51.2F5 cells were
cultured in a-MEM with 10% FBS and 36 ng/mL of Human recombinant
CSF-1 (a gift from Chiron Corp., Emeryville, CA). MTLn3-GFP (n = 50,00