Technical Reference #991

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35 mm glass bottom microwell dishes 155 that were poly-D-lysine coated (MatTek cultureware; Ashland; MA)

991.

Fragile X Mental Retardation Protein Fmrp Binds Mrnas In The Nucleus Miri Kim; Michel Bellini; and Stephanie Ceman, University of Illinois Urbana-Champaign, Molecular and Cellular Biology, 29(991), (2009)
Link To Paper

Abstract:
The Fragile X mental retardation protein (FMRP) is an RNA binding protein that associates with a large collection of mRNAs. Since FMRP was previously shown to be a nucleocytoplasmic shuttling protein we examined the hypothesis that FMRP binds its cargo m

Materials & Methods:
For live imaging by confocal microscopy the cells were plated at 2.5 105 cells/ml on 35-mm glass-bottom microwell dishes that were poly-D-lysine-coated (MatTek Cultureware Ashland MA).

Microscopic Technique
Confocal Microscopy, Live cell

Cell Type(s)
COS-7