Technical Reference #946

946.

Vimentin Filaments Support Extension Of Tubulin-Based Microtentacles In Detached Breast Tumor Cells. Rebecca A. Whipple; Eric M. Balzer; Edward H. Cho; Michael A. Matrone; Jennifer R. Yoon; and Stuart S. Martin, University of Maryland, Cancer Research, 68(946), (2008)
Link To Paper

Abstract:
Solid tumor metastasis often involves detachment of epithelial carcinoma cells into the vasculature or lymphatics. However most studies of cytoskeletal rearrangement in solid tumors focus on attached cells. In this study we report for the first time tha

Keywords:
metastasis; breast cancer; tubulin; microtentacles; vimentin; cytokeratin

Materials & Methods:
Differential interference contrast (DIC) images of MDA-MB-436 cells suspended over 35-mm glass-bottomed dishes (MatTek) were acquired using a Zeiss Axiovert 200M motorized microscope

Microscopic Technique
Optical Microscopy, Differential Interference Contrast Microscopy

Cell Type(s)
MDA-MB-436