Technical Reference #943

943.

Evidence Of A Continuous Endoplasmic Reticulum In The Protozoan Parasite Entamoeba Histolytica Jose E. Teixeira and Christopher D. Huston, University of Vermont, Eukaryotic Cell, 7(943), (2008)
Link To Paper

Abstract:
Entamoeba histolytica the cause of amebiasis is believed to have no continuous endoplasmic reticulum (ER) with ER functions occurring in vesicles. Here using an ER-targeted green fluorescent protein fusion protein and fluorescence loss in photobleachi

Keywords:
endoplasmic reticulum; protozoan; amebiasis; evolution

Materials & Methods:
Trophozoites adherent to glass-bottomed culture dishes (MatTek Ashland MA) were covered with a thin layer of Luria-Bertani (LB) top agar to limit parasite movement and were examined using a Zeiss 510 META confocal microscope.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Trophozoites