Technical Reference #936

936.

Hydrophobic Inactivation Of Influenza Viruses Confers Preservation Of Viral Structure With Enhanced Immunogenicity Yossef Raviv; Robert Blumenthal; S. Mark Tompkins; Jennifer Humberd; Robert J. Hogan; and Mathias Viard, National Cancer Institute, The Journal of Virology, 82(936), (2008)
Link To Paper

Abstract:
The use of inactivated influenza virus for the development of vaccines with broad heterosubtypic protection requires selective inactivation techniques that eliminate viral infectivity while preserving structural integrity. Here we tested if a hydrophobic

Keywords:
influenza; INA; hydrophobic inactivation; transmembrane proteins

Materials & Methods:
FRAP: HeLa cells were plated on 35-mm glass-bottom dishes (MatTek Ashland MA) and either transfected with CD4-GFP 24 h prior to confocal analysis as described previously (30) or labeled with DiO 1 hour prior to the FRAP analysis.

Microscopic Technique
Fluorescence Microscopy, Fluorescence Redistribution After Photobleaching

Cell Type(s)
HeLa