Technical Reference #935

935.

Organellar Dynamics During The Cell Cycle Of Manami Nishi; Ke Hu; John M. Murray and David S. Roos, University of Pennsylvania, The Journal of Cell Science, 121(935), (2008)
Link To Paper

Abstract:
The protozoan phylum Apicomplexa encompasses ~5000 species of obligate intracellular parasites including those responsible for malaria and toxoplasmosis. Rather than dividing by binary fission apicomplexans use a remarkable mechanism for replication as

Keywords:
Apicomplexan parasites; Organelle segregation; Apicoplast; Mitochondrion; Endoplasmic reticulum; Golgi; Centrioles; Micronemes; Rhoptries

Materials & Methods:
For time-lapse microscopy 3-5 x 10^5 parasites were inoculated into confluent HFF cell monolayers grown in glass-bottomed dishes (MatTek) and incubated at 37°C for 16-24 hours.

Microscopic Technique
Fluorescence Microscopy, Time Lapse

Cell Type(s)
HFF