Technical Reference #933

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35 mm dishes with glass coverslip bottoms (MatTek Corp.; MA)

933.

Human Ddx3 Functions In Translation And Interacts With The Translation Initiation Factor Chung-Sheng Lee; Anusha P. Dias; Mark Jedrychowski; Arvind H. Patel; Jeanne L. Hsu and Robin Reed, Harvard Medical School, Nucleic Acids Research, 36(933), (2008)
Link To Paper

Abstract:
The conserved RNA helicase DDX3 is of major medical importance due to its involvement in numerous cancers human hepatitis C virus (HCV) and HIV. Although DDX3 has been reported to have a wide variety of cellular functions its precise role remains obscur

Keywords:
DDX3; protein translation; RNA helicase

Materials & Methods:
RNA interference: HeLa cells were plated overnight on 35mm dishes with glass coverslip bottoms (MatTek Corp. MA) and transfected with lipofectamine 2000 (Invitrogen) and 50nM siRNA according to the manufacturer’s protocol.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HeLa