Technical Reference #929

929.

Impaired Insulin Secretion By Diphenyleneiodium Associated With Perturbation Of Cytosolic Ca2+ Dynamics In Pancreatic 5 Β Cells Hirofumi Imoto; Nobuhiro Sasaki; Masanori Iwase; Udai Nakamura; Miwako Oku; Kazuo Sonoki; Yuji Uchizono; Mitsuo Iida, Kyushu University, Endocrinology, 149(929), (2008)
Link To Paper

Abstract:
Pancreatic islets express superoxide-producing NADPH oxidase system but its role remains unknown. To address this we studied the mechanisms of impaired insulin secretion induced by diphenyleneiodium (DPI) an NADPH-oxidase inhibitor. We investigated the

Keywords:
Diabetes mellitus; RIN cells; islets of Langerhans; NADPH oxidase; mitochondria

Materials & Methods:
Islets were attached to a 275 glass-bottom culture dish (MatTek Ashland MA) precoated with 100 μg/ml poly-L-lysine (Sigma-Aldrich)

Microscopic Technique
Fluorescence Microscopy, Calcium Imaging

Cell Type(s)
Islet