Technical Reference #926

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
Multiwell glass bottom Petri dishes (MatTek Corporation; Ashland; MI)

926.

Derivatized 2-Furoyl-Ligrlo-Amide; A Versatile And Selective Probe For Proteinase-Activated Receptor 2: Binding And Visualization Morley D. Hollenberg; Bernard Renaux; Eric Hyun; Steeve Houle; Nathalie Vergnolle; Mahmoud Saifeddine; and Rithwik Ramachandran, University of Calgary, Journal of Pharmacology and Experimental Therapeutics, 326(926), (2008)
Link To Paper

Abstract:
The proteinase-activated receptor-2 (PAR2)-activating peptide with an N-terminal furoyl group modification 2-furoyl-LIGRLO-NH2 (2fLI) was derivatized via its free ornithine amino group to yield [3H]propionyl-2fLI and Alexa Fluor 594-2fLI that were used

Keywords:
PAR; ligand probe; Alexa Fluor

Materials & Methods:
Cells to be used for fluorescence microscopy were similarly resuspended from the T-flasks reseeded in Multiwell glass bottom Petri dishes (MatTek Corporation Ashland MI) and grown to approximately 60% confluence before use.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
KNRK