Technical Reference #925

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
A-coated 24-well glass bottom dishes (MatTek)

925.

Cdt1 And Cdc6 Are Destablized By Rereplication-Induced Dna Damage Jonathan R. Hall; Hyun O. Lee; Brandon D. Bunker; Elizabeth S. Dorn; Greg C. Rogers; Robert J. Duronio; and Jeanette Gowen Cook, University of North Carolina Chapel Hill, The Journal of Biological Chemistry, 283(925), (2008)
Link To Paper

Abstract:
The replication factors Cdt1 and Cdc6 are essential for origin licensing a prerequisite for DNA replication initiation. Mechanisms to ensure that metazoan origins initiate once per cell cycle include degradation of Cdt1 during S phase and inhibition of C

Keywords:
DNA replication; Cdt1; Cdc6; geminin; rereplication

Materials & Methods:
Drosophila cells were transfected then plated in concanavalin A-coated 24-well glass-bottom dishes (MatTek) for 1 hr prior to fixation as described

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Drosophila Schneider 2 (S2)