Technical Reference #910

910.

Nf-Kb Signalling Is Inhibited By Glucocorticoid Receptor And Stat6 Via Distinct Mechanisms Glyn Nelson; Geraint J. C. Wilde; David G. Spiller; Stephnie M. Kennedy; David W. Ray; Elaine Sullivan; John F. Unitt and Michael R. H. White, University of Liverpool, The Journal of Cell Science, 116(910), (2003)
Link To Paper

Abstract:
NF-kB transcription factors are involved in the cellularresponse to stress and are regulated by inhibitor (IkB)proteins which prevent NF-kB-mediated transcription bymaintaining NF-kB in the cytoplasm. Proteins from otherpathways are also known t

Keywords:
NF-kB; STAT6; Glucocorticoid receptor; Signal transduction; Fluorescent protein fusions; Confocal microscopy

Materials & Methods:
For confocal microscopy cells were plated on 35 mm Mattek dishes (Mattek USA) at 2´104 cells per plate in 2 ml medium.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa