Technical Reference #905

905.

Ku80 Participates In The Targeting Of Retroviral Transgenes To The Chromatin Of Cho Cells Christel Masson;1 Ste´phanie Bury-Mone´;1† Elvire Guiot;1 Asier Saez-Cirion;2 Damien Schoe¨vae¨rt-Brossault;3;4 Corinne Brachet-Ducos;1 Olivier Delelis;1 Fre´de´ric Subra;1 Laurence Jeanson-Leh;1‡ and Jean-Franc¸ois Mouscadet, Institut Pasteur, The Journal of Virology, 81(905), (2007)
Link To Paper

Abstract:
The heterodimer Ku70/80 Ku is the DNA-binding component of the DNA-PK complex required for thenonhomologous end-joining pathway. It participates in numerous nuclear processes including telomereand chromatin structure maintenance replication and tra

Materials & Methods:
A total of 2 104 cells were plated on the center of glass-bottom dishes (MatTek Corp.) and infected with 4.2 ng of VSV-G pseudotyped HIV-1 particles bearing the luciferase reporter gene.

Microscopic Technique
Luminescence Microscopy

Cell Type(s)
CHO