Technical Reference #904

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
MatTek glass bottom microwells (MatTek; Ashland; MA)

904.

Live Cell Dynamics Of Pml Nuclear Bodies Upon Entry Into And Exit From Mitosis Yi-Chun M. Chen; Constantin Kappel; Joel Beaudouin; Roland Eils; and David L. Spector, Stony Brook University, Molecular Biology of the Cell, 19(904), (2008)
Link To Paper

Abstract:
Promyelocytic leukemia nuclear bodies (PML NBs) have been proposed to beinvolved in tumor suppression viral defense DNA repair and/or transcriptionalregulation. To study the dynamics of PML NBs during mitosis we developedseveral U2OS cell lines

Materials & Methods:
Cells were seeded onto MatTek glass bottom microwells and grown for 2 days before imaging.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
U2OS