Technical Reference #898

898.

Whole Genome Functional Analysis Identifies Novel Components Required For Mitotic Spindle Integrity In Human Cells Daniel R Rines; Maria Ana Gomez-Ferreria; Yingyao Zhou; Paul DeJesus; Seanna Grob; Serge Batalov; Marc Labow; Dieter Huesken; Craig Mickanin; Jonathan Hall; Mischa Reinhardt; Francois Natt; Joerg Lange; David J Sharp; Sumit Chanda; Jeremy S Caldwell, Genomics Institute of the Novartis Research Foundation, Genome Biology, 9(898), (2008)
Link To Paper

Abstract:
Background: The mitotic spindle is a complex mechanical apparatus required for accuratesegregation of sister chromosomes during mitosis. We designed a genetic screen using automatedmicroscopy to discover factors essential for mitotic progression. Usin

Materials & Methods:
In brief 80 nmol of the siRNA was diluted in 500 μl of OptiMem (Invitrogen) and placed in a 35 mm glass bottom dish (MatTek Corporation Ashland MA).

Microscopic Technique
Confocal Microscopy, Spinning Disc

Cell Type(s)
HeLa