Technical Reference #895

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass bottom Petri dishes (MatTek Corp.; Ashland; MA)

895.

A Truncated P2X7 Receptor Variant (P2X7-J) Endogenously Expressed In Cervical Cancer Cells Antagonizes The Full-Length P2X7 Receptor Through Hetero-Oligomerization Ying-Hong Feng; Xin Li; Liqin Wang; Lingying Zhou; and George I. Gorodeski, University of the Health Sciences, The Journal of Biological Chemistry, 281(895), (2006)
Link To Paper

Abstract:
A truncated naturally occurring variant of the human receptor P2X7 was identified in cancer cervicalcells. The novel protein (P2X7-j) a polypeptide of 258 amino acids lacks the entire intracellularcarboxyl terminus the second transmembrane domain

Materials & Methods:
Briefly cells were seeded at 2–3 × 105 on 35-mm glass bottom Petri dishes (MatTek Corp. Ashland MA) and allowed to reach confluence.

Microscopic Technique
Confocal Microscopy, Dynamic Laser Scanning

Cell Type(s)
CaSki, HeLa, SiHa