Technical Reference #893

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
MatTek dishes

893.

The Conformational State Of Tes Regulates Its Zyxin-Dependent Recruitment To Focal Adhesions Boyan K. Garvalov; Theresa E. Higgins; James D. Sutherland; Markus Zettl; Niki Scaplehorn; Thomas Köcher; Eugenia Piddini; Gareth Griffiths; and Michael Way, European Molecular Biology Laboratory, The Journal of Cell Biology, 161(893), (2003)
Link To Paper

Abstract:
he function of the human Tes protein which hasextensive similarity to zyxin in both sequence anddomain organization is currently unknown. We nowshow that Tes is a component of focal adhesions that whenexpressed negatively regulates proliferati

Keywords:
Tes; focal adhesion; zyxin; RNAi; conformation

Materials & Methods:
10 5 viable T47D cells/ml (wild-type or stably expressing GFP-Tes or GFP) were plated onto fibronectin (10 g/ml) coated MatTek dishes.

Microscopic Technique
Video Microscopy

Cell Type(s)
T47D