Technical Reference #892

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass-bottom dishes (MatTek)

892.

Maintenance Of Self-Renewal Ability Of Mouse Embryonic Stem Cells In The Absence Of Dna Methyltransferases Dnmt1; Dnmt3A And Dnmt3B Akiko Tsumura ; Tomohiro Hayakawa ; Yuichi Kumaki ; Shin-ichiro Takebayashi; Morito Sakaue; ; Chisa Matsuoka ; Kunitada Shimotohno ; Fuyuki Ishikawa ; En Li; Hiroki R. Ueda ; Jun-ichi Nakayama; Masaki Okano, RIKEN Developmental Biology Japan; Kyoto University Japan; INTEC Web and Genome Informatics Corp. Japan; Novartis Institute for Biomedical Research, Genes to Cells, 11(892), (2006)
Link To Paper

Abstract:
DNA methyltransferases Dnmt1 Dnmt3a and Dnmt3b cooperatively regulate cytosine methylationin CpG dinucleotides in mammalian genomes providing an epigenetic basis for gene silencingand maintenance of genome integrity. Proper CpG methylation is requir

Materials & Methods:
Wild-type and knockout ES cells were cultured in 35-mm glass-bottom dishes (MatTek) precoated with 0.2% gelatin in PBS.

Microscopic Technique
Fluorescence Microscopy, Immunofluorescence

Cell Type(s)
hESCs