Technical Reference #886

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottom dish (MatTek Co.; Ashland; MA; USA)

886.

Calpain-Mediated Regulation Of The Distinct Signaling Pathways And Cell Migration In Human Neutrophils Masataka Katsube; Takayuki Kato; Maki Kitagawa; Haruyoshi Noma; Hisakazu Fujita; and Seiichi Kitagawa, Osaka City University, Journal of Leukocyte Biology, 84(886), (2008)
Link To Paper

Abstract:
We studied the mechanisms underlyingcalpain inhibition-mediated human neutrophil migration.MAPKs including ERK p38 and JNKMEK1/2 MAPK kinase 3/6 (MKK3/6) PI-3K/Aktc-Raf and p21-activated kinase (PAK; an effectormolecule of Rac) were rapi

Keywords:
Mitogen-activated protein kinases; pertussis toxin phosphatidylinositol 3-kinase; Rac

Materials & Methods:
Cells (3.5105/ml) suspended in HBSS were placed in a glass-bottom dish (MatTek Co. Ashland MA USA)

Microscopic Technique
Time Lapse Microscopy

Cell Type(s)
HL-60