Technical Reference #884

884.

Mdia2 Induces The Actin Scaffold For The Contractile Ring And Stabilizes Its Position During Cytokinesis In Nih 3T3 Cells Sadanori Watanabe; Yoshikazu Ando; Shingo Yasuda; Hiroshi Hosoya; Naoki Watanabe; Toshimasa Ishizaki and Shuh Narumiya, Kyoto University University Japan, Molecular Biology of the Cell, 19(884), (2008)
Link To Paper

Abstract:
mDia proteins are mammalian homologs of Drosophila diaphanous and belong to theformin family proteins that catalyze actin nucleation and polymerization. While forminfamily proteins of nonmammalian species such as Drospohila diaphanous are essential in

Keywords:
mDia2; Actin Scaffold; Contractile Ring; NIH 3T3; Cytokinesis

Materials & Methods:
NIH 3T3 cells were seeded on 35-mm glass-bottom dishes (Mat Tek) with or without siRNA transfection.

Microscopic Technique
Time Lapse Microscopy

Cell Type(s)
3T3