Technical Reference #881

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-l-lysine–coated glass-bottom dishes (MatTek; Ashland; MA; USA) glass-bottomed dishes

881.

Hydroxylation Increases The Neurotoxic Potential Of Bde-47 To Affect Exocytosis And Calcium Homeostasis In Pc12 Cells Milou M.L. Dingemans; Aart de Groot; Regina G.D.M. van Kleef; Åke Bergman; Martin van den Berg; Henk P.M. Vijverberg; and Remco H.S. Westerink, Utrecht University, Environmental Health Perspectives, 116(881), (2008)
Link To Paper

Abstract:
Oxidative metabolism resulting in the formation of hydroxylated polybrominateddiphenyl ether (PBDE) metabolites may enhance the neurotoxic potential of brominated flameretardants.OBJECTIVE: Our objective was to investigate the effects of a hydroxy

Keywords:
Bioactivation; brominated flame retardants; calcium; catecholamine; exocytosis; intracellular calcium stores; neurotoxicity; neurotransmitter release; persistent organic pollutants; polybrominated diphenyl ether.

Materials & Methods:
For Ca2+ imaging experiments we subcultured undifferentiated PC12 cells in poly-L-lysine–coated glass-bottom dishes (MatTek Ashland MA

Microscopic Technique
Fluorescence Microscopy, Calcium Imaging

Cell Type(s)
PC12