Technical Reference #878

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35 mm Petri dishes with 13 mm glass inserts (MatTek Corporation)

878.

The Integrity Of A Lamin-B1-Dependent Nucleoskeleton Is A Fundamental Determinant Of Rna Synthesis In Human Cells Chi W. Tang; Apolinar Maya-Mendoza; Catherine Martin; Kang Zeng; Songbi Chen; Dorota Feret; Stuart A. Wilson and Dean A. Jackson, University of Manchester, The Journal of Cell Science, 121(878), (2008)
Link To Paper

Abstract:
Spatial organisation of nuclear compartments is an importantregulator of chromatin function yet the molecular principlesthat maintain nuclear architecture remain ill-defined. We haveused RNA interference to deplete key structural nuclearproteins

Keywords:
Nuclear lamin proteins; Nucleoskeleton; Nuclear organisation; RNA synthesis; Chromosome territories

Materials & Methods:
For live imaging of CTs labelled cells were grown on 35 mm Petri dishes with 13 mm glass inserts (MatTek Corporation) in medium without Phenol Red.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa