Technical Reference #874

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass culture dishes (MatTek Corp; Ashland; MA)

874.

Design Of Mutant Β2 Subunits As Decoy Molecules To Reduce The Expression Of Functional Ca2 Channels In Cardiac Cells Sabine Telemaque; Swapnil Sonkusare; Terrie Grain; Sung W. Rhee; Joseph R. Stimers; Nancy J. Rusch; and James D. Marsh, University of Arkansas, Journal of Pharmacology and Experimental Therapeutics, 325(874), (2008)
Link To Paper

Abstract:
Calcium influx through long-lasting (“L-type”) Ca2 channels(CaV) drives excitation-contraction in the normal heart. Dysregulationof this process contributes to Ca2 overload andinterventions that reduce expression of the pore-forming 1subunit may

Keywords:
Functional Ca2+ Channels; Mutant B2; Cardiac Cells

Materials & Methods:
After trypsinization dissociated cells were plated in glass culture dishes (MatTek Ashland MA) for electrophysiology (60–80 103 cells/ml).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HL-1