Technical Reference #872

872.

Mob4 Plays A Role In Spindle Focusing In Drosophila S2 Cells Matthew A. Trammell; Nicole M. Mahoney; David A. Agard and Ronald D. Vale, University of California San Francisco (UCSF), The Journal of Cell Science, 121(872), (2008)
Link To Paper

Abstract:
The characteristic bipolar shape of the mitotic spindle is produced by the focusing of the minus ends of microtubules at the spindle poles. The focus is maintained by the centrosome a microtubule-nucleating organelle as well as by proteins that are capa

Keywords:
RNAi; Centrosome; Microtubule; Kinetochore; -tubulin

Materials & Methods:
Cells were adhered on Con-A treated glass-bottom culture dishes (35-mm MatTek)

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Drosophila Schneider 2 (S2)