Technical Reference #871

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
sterile glass-bottomed culture dishes (MatTek Corporation; Ashland; MA)

871.

Dextran Microgels For Time-Controlled Delivery Of Sirna Koen Raemdonck ; Tinneke G. Van Thienen ; Roosmarijn E. Vandenbroucke ; Niek N. Sanders; Joseph Demeester ; Stefaan C. De Smedt, Ghent University Harelbekestraat, Advanced Functional Materials, 18(871), (2008)
Link To Paper

Abstract:
To apply siRNA as a therapeutic agent appropriate attention should be paid to the optimization of the siRNA gene silencing effect both in terms of magnitude and duration. Intracellular time-controlled siRNA delivery could aid in tailoring the kinetics o

Keywords:
Biodegradable materials • Controlled release • Microgels • siRNA • Drug delivery • Biomedical applications

Materials & Methods:
HUH7 cells were seeded in sterile glass-bottomed culture dishes (MatTek Corporation Ashland MA) at a density of 3 104 cells per cm2 and allowed to attach overnight.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa