Technical Reference #870

870.

A 20 Nm Step Toward The Cell Membrane Preceding Exocytosis May Correspond To Docking Of Tethered Granules Erdem Karatekin;Viet Samuel Tran; Se´bastien Huet; Isabelle Fanget; Sophie Cribiery and Jean-Pierre Henry, Université Paris, Biophysical Journal, 94(870), (2008)
Link To Paper

Abstract:
In endocrine cells plasma membrane (PM)-bound secretory granules must undergo a number of maturation stages(i.e. priming) to become fusion-competent. Despite identification of several molecules involved in binding granules to the PM andpriming them

Keywords:
Exocytosis; endocrine cells; image analysis; secretory granule-plasma membrane interactions; tethering factors; total internal reflection fluorescence microscopy

Materials & Methods:
After transfection cells were plated onto uncoated glass-bottom dishes (P50G-1.5-14-F MatTek Cultureware Ashland MA).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
BON