Technical Reference #868

868.

Setting Clock Speed In Mammals: The Ck1 Tau Mutation In Mice Accelerates Circadian Pacemakers By Selectively Destabilizing Period Proteins Qing-Jun Meng; Larisa Logunova; Elizabeth S. Maywood; Monica Gallego; Michael H. Hastings; Andrew S.I. Loudon, University of Manchester, Neuron, 58(868), (2008)
Link To Paper

Abstract:
The intrinsic period of circadian clocks is their defining adaptive property. To identify the biochemical mechanisms whereby casein kinase1 (CK1) determines circadian period in mammals we created mouse null and tau mutants of Ck1 epsilon. Circadian perio

Keywords:
CK1 tau Mutation in Mice; Circadian Pacemakers

Materials & Methods:
At 80%–90% confluence in glass-bottomed dishes (MatTek) cells were transfected with combinations of relevant plasmids using 3 μl of GeneJuice (Novagen).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
3T3