Technical Reference #864

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottomed dishes (MatTek, Ashland, MA)

864.

Simultaneous Nitric Oxide And Dehydroascorbic Acid Imaging By Combining Diaminofluoresceins And Diaminorhodamines Xiaoying Ye, Stanislav S. Rubakhin, Jonathan V. Sweedler, University of Illinois, The Journal of Neuroscience Methods, 168(864), R141, (2008)
Link To Paper

Abstract:
Spatial measurements of nitric oxide (NO) production are important to understand the function and metabolism of this molecule. The reagent, 4,5-diaminofluorescein (DAF-2) and several structurally similar probes are widely used for detection and imaging

Keywords:
Nitric oxide; Dehydroascorbic acid; Fluorescence imaging; DAF-2 DA; DAR-4M AM; PC12 cells

Materials & Methods:
Cells were transferred and cultured in glass-bottomed dishes (MatTek, Ashland, MA)

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
PC12