Technical Reference #864

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottomed dishes (MatTek; Ashland; MA)

864.

Simultaneous Nitric Oxide And Dehydroascorbic Acid Imaging By Combining Diaminofluoresceins And Diaminorhodamines Xiaoying Ye; Stanislav S. Rubakhin; Jonathan V. Sweedler, University of Illinois, The Journal of Neuroscience Methods, 168(864), (2008)
Link To Paper

Abstract:
Spatial measurements of nitric oxide (NO) production are important to understand the function and metabolism of this molecule. The reagent 45-diaminofluorescein (DAF-2) and several structurally similar probes are widely used for detection and imaging

Keywords:
Nitric oxide; Dehydroascorbic acid; Fluorescence imaging; DAF-2 DA; DAR-4M AM; PC12 cells

Materials & Methods:
Cells were transferred and cultured in glass-bottomed dishes (MatTek Ashland MA)

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
PC12