Technical Reference #863

863.

Neurofibromin Is Actively Transported To The Nucleus Ina Vandenbroucke; Patrick Van Oostveldt; Elisabeth Coene ; Anne De Paepe and Ludwine Messiaen, Ghent University Hospital, FEBS Letters, 560(863), (2004)

Abstract:
Mutations in the neurofibromatosis type 1 (NF1) tumor suppressor gene predispose individuals to a variety of benign and malignant tumors. Many tumor suppressors ‘shuttle’ between the nucleus and the cytoplasm thus regulating their function. By expressing

Keywords:
Neurofibromatosis type 1; Neurofibromin; Nuclear localization signal; Nuclear localization; Alternative splicing

Materials & Methods:
For live cell imaging and photobleaching experiments cells were plated transfected and observed in glass bottom culture dishes (MatTek Corporation)

Microscopic Technique
Confocal Microscopy

Cell Type(s)
COS-7, PLC/PRF/5 cells