Technical Reference #861

861.

Non-Invasive Characterization Of Structure And Morphology Of Silk Fibroin Biomaterials Using Non-Linear Microscopy William L. Rice; Shamaraz Firdous; Sharad Gupta; Martin Hunter a; Cheryl W.P. Foo a; Yongzhong Wang a; Hyeon Joo Kim a; David L. Kaplan a; Irene Georgakoudi, Tufts University, Biomaterials, 29(861), (2008)
Link To Paper

Abstract:
Designing biomaterial scaffolds remains a major challenge in tissue engineering. Key to this challenge is improved understanding of therelationships between the scaffold properties and its degradation kinetics as well as the cell interactions and the p

Keywords:
Silk; Fibroin; Two photon excited fluorescence; Second harmonic generation; Spectral analysis; Non-invasive characterization

Materials & Methods:
Silk fibroin films were made by pipetting volumes of aqueous or HFIP silk solutions onto 35 mm glass bottom dishes (MatTek Acton MA)...Samples were placed on culture dishes with number 1.5 cover glass bottoms (MATTEK Ashland MA)

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
mesenchymal