Technical Reference #858

858.

Immunolabeling For Correlative Light And Electron Microscopy On Ultrathin Cryosections Irawati K. Kandela; Reiner Bleher; and Ralph M. Albrecht, University of Wisconsin, Microscopy and Microanalysis, 14(858), (2008)
Link To Paper

Abstract:
Correlative labeling permits colocalization of molecular species for observation of the same sample inlight ~LM! and electron microscopy ~EM!. Myosin bands in ultrathin cryosections were labeled using bothfluorophore conjugated to secondary antibody ~

Keywords:
Correlative labeling; immunofluorescence; immuno-EM; colloidal gold particles; fluorophores; cryosections; nanoparticles

Materials & Methods:
After washing the grids were placed face down in 3–5 mm glass bottom microwell petri dishes ~MatTek P35G MatTek Corp. Ashland MA!. These dishes have a #1.5 coverslip as part of the bottom. Thus

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Skeletal muscle tissue