Technical Reference #856

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm microwell plastic dishes with glass coverslips glued to 1-cm diameter holes in the center of each dish (Mattek Corp.; Ashland; MA)

856.

Regulation Of Intracellular Ph In Human Melanoma: Potential Therapeutic Implications Miriam L. Wahl;2 Judith A. Owen; Randy Burd; Robin A. Herlands; Suzanne S. Nogami; Ulrich Rodeck; David Berd; Dennis B. Leeper; and Charles S. Owen, Haverford College, Molecular Cancer Therapeutics, 1(856), (2002)
Link To Paper

Abstract:
Melanoma cells in vivo maintain intracellular pH (pHi) ina viable range despite an extracellular tumor pH (pHe)that is typically below 7.0. In general three families oftransporters are capable of removing metabolicprotons but the specific transp

Keywords:
Melanoma

Materials & Methods:
Cells were plated on 35-mm microwell plastic dishes with glass coverslips glued to 1-cm diameter holes in the center of each dish (Mattek Corp. Ashland MA).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Sk-Mel-28