Technical Reference #854

854.

Semi-Automated Quantification Of Filopodial Dynamics Santiago Costantino;; Christopher B. Kent; Antoine G. Godin; Timothy E. Kennedy; PaulW.Wiseman; Alyson E. Fournier, McGill University, The Journal of Neuroscience Methods, 171(854), (2008)
Link To Paper

Abstract:
Cellular motility underlies critical physiological processes including embryogenesis metastasis andwound healing. Nerve cells undergo cellular migration during development and also extend neuronalprocesses for long distances through a complex microen

Keywords:
Growth cone dynamics; Filopodia dynamics; Single particle tracking Filopodia tracking; Automated quantification fluorescence microscopy Image analysis

Materials & Methods:
explants are dissected from E13 chicks and cultured in growth media (F-12 supplemented with 10% Fetal Bovine Serum and 50 ng/l of 7S nerve growth factor) on glass-bottomed culture dishes (MatTek Co. Ashland MA)

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
E13, Chick dorsal root ganglion