Technical Reference #853

853.

Cellular Trafficking Of Lipoteichoic Acid And Toll-Like Receptor 2 In Relation To Signaling; Role Of Cd14 And Cd36 Nadra J. Nilsen; Susanne Deininger; Unni Nonstad; Frode Skjeldal; Harald Husebye; Dmitrii Rodionov; Sonja von Aulock; Thomas Hartung; Egil Lien; Oddmund Bakke; and Terje Espevik, Norwegian University of Science and Technology, Journal of Leukocyte Biology, 84(853), (2008)
Link To Paper

Abstract:
Lipoteichoic acid (LTA) is a central inducerof inflammatory responses caused by Grampositivebacteria such as Staphylococcus aureusvia activation of TLR2. Localization of TLR2 inrelation to its coreceptors may be important forfunction. This stu

Keywords:
TLR; LTA; endocytosis

Materials & Methods:
MDCK cells stably expressing Eea-1GFP were transiently transfected with the TLR2 overnight in Microwell dishes for microscopy (MatTek Corp.)

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293, MDCK