Technical Reference #841

841.

Heterogeneity And Timing Of Translocation And Membrane-Mediated Assembly Of Different Annexins Tatsiana Skrahina; Alen Piljić1; Carsten Schultz, European Molecular Biology Laboratory, Experimental Cell Research, 314(841), (2008)
Link To Paper

Abstract:
Many cell types including neurons and epithelial cells express a variety of annexins.Although the overall function has only been partially unravelled a dominant feature is theformation of two-dimensional assemblies under the plasma membrane in a ca

Keywords:
Annexin; Calcium binding; FRAP; FRET; Translocation

Materials & Methods:
For imaging experiments cells were plated in 35 mm MatTek chambers (Ashland MA) and transfected with FuGENE 6 reagent (Roche Mannheim Germany) at 50% confluency.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
N1E-115