Technical Reference #840

840.

A New Role For Cortactin In Invadopodia: Regulation Of Protease Secretion Emily S. Clark; Alissa M. Weaver, Vanderbilt University, European Journal of Cell Biology, 87(840), (2008)

Abstract:
Invadopodia are actin-dependent organelles that function in the invasion and remodeling of the extracellular matrix(ECM) by tumor cells. Cortactin a regulator of the Arp2/3 complex is of particular importance in invadopodiafunction. While most of th

Keywords:
Cortactin; Invadopodia; Matrix metalloproteinase; Protease; Membrane trafficking; Vesicle

Materials & Methods:
To coat MatTek dishes 2.5% gelatin/2.5% sucrose in PBS was heated to 37 1C and added to the dish followed by

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HNSCC