Technical Reference #835

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35mmdishes (MatTeKCorporation;USA)

835.

Sarcoplasmic Reticulum Ca2+ Release Channel Ryanodine Receptor (Ryr2) Plays A Crucial Role In Aconitine-Induced Arrhythmias Min Fu a;b; Ru-Xin Li a; Li Fan c; Guo-Wei He c; Kent L. Thornburg d;**; Zhao Wang, Tsinghua University, Biochemical Pharmacology, 75(835), (2008)

Abstract:
The present study established a model of RyR2 knockdown cardiomyocytes and elucidatedthe role of RyR2 in aconitine-induced arrhythmia. Cardiomyocytes were obtained fromhearts of neonatal Sprague–Dawlay rats. siRNAs were used to down-regulate RyR2 expr

Keywords:
RyR2; Knockdown; Aconitine; Arrhythmia; Excitation–contraction coupling

Materials & Methods:
Cardiomyocytes were inoculated in 35mmdishes (MatTeKCorporationUSA) and loaded with 5 mM fluo-3 AM (Sigma Chemical Co. USA).

Microscopic Technique
Fluorescence Microscopy, Immunofluorescence

Cell Type(s)
Cardiac myocytes