Technical Reference #834

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35mm2 glass coverslip dishes coated with poly-l-lysine (MatTek Corp.)

834.

The Kinesin-8 Motor Kif18A Suppresses Kinetochore Movements To Controlmitoticchromosomealignment Jason Stumpff;1 George von Dassow;2 Michael Wagenbach;1 Charles Asbury;1 and Linda Wordeman, University of Washington, Developmental Cell, 14(834), (2008)
Link To Paper

Abstract:
During vertebrate cell division chromosomes oscillatewith periods of smooth motion interrupted byabrupt reversals in direction. These oscillationsmust be spatially constrained in order to align andsegregate chromosomes with high fidelity but the

Keywords:
Kinesin-8 Motor Kif18A; Kinetochore Movements; MitoticChromosomeAlignment

Materials & Methods:
HeLa cells were cultured in MEMalpha (Life Technologies) medium with 10% FBS (Hyclone) at 37C and 5% CO2 on 35 mm2 glass coverslip dishes coated with poly-l-lysine (MatTek

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa