Technical Reference #832

832.

A Multi-Component Assembly Pathway Contributes To The Formation Of Acentrosomal Microtubule Arrays In Interphase Drosophila Cells Gregory C. Rogers, Nasser M. Rusan, Mark Peifer* and Stephen L. Rogers, University of North Carolina, Chapel Hill, Molecular Biology of the Cell, 19(832), 3164, (2008)
Link To Paper

Abstract:
In animal cells, centrosomes nucleate microtubules that form polarized arrays to organize the cytoplasm.Drosophila presents an interesting paradox however, as centrosome-deficient mutant animals developinto viable adults. To understand this discrepanc

Keywords:
Drosophila, Acentrosomal Microtubule Arrays

Materials & Methods:
Stable cell lines were generated using Effectene transfection reagent (Qiagen)/pCoHygro selection system (Invitrogen) and plated on 0.5mg/ml conA-coated glass-bottom dishes (MatTek) for 1hr prior to observation.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Drosophila, RNAi, ML-DmD16-c3