Technical Reference #832

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
0.5mg/ml conA-coated glass-bottom dishes (MatTek)

832.

A Multi-Component Assembly Pathway Contributes To The Formation Of Acentrosomal Microtubule Arrays In Interphase Drosophila Cells Gregory C. Rogers; Nasser M. Rusan; Mark Peifer* and Stephen L. Rogers, University of North Carolina Chapel Hill, Molecular Biology of the Cell, 19(832), (2008)
Link To Paper

Abstract:
In animal cells centrosomes nucleate microtubules that form polarized arrays to organize the cytoplasm.Drosophila presents an interesting paradox however as centrosome-deficient mutant animals developinto viable adults. To understand this discrepanc

Keywords:
Drosophila; Acentrosomal Microtubule Arrays

Materials & Methods:
Stable cell lines were generated using Effectene transfection reagent (Qiagen)/pCoHygro selection system (Invitrogen) and plated on 0.5mg/ml conA-coated glass-bottom dishes (MatTek) for 1hr prior to observation.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Drosophila, RNAi, ML-DmD16-c3