Technical Reference #828

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
MatTek 3.5-mm Petri dishes

828.

Opposing Roles Of Syndecan-1 And Syndecan-2 In Polyethyleneimine-Mediated Gene Delivery Se´bastien Paris; Alina Burlacu; and Yves Durocher, Biotechnology Research Institute, The Journal of Biological Chemistry, 283(828), (2008)
Link To Paper

Abstract:
Polyethyleneimines (PEIs) are efficient non-viral vectors for gene transfer. Heparan sulfate proteoglycans have been proposedto be the cell-surface receptors for PEIDNA complexes(polyplexes). Here we investigated if syndecan-1 (SDC1) andsyndecan-2

Materials & Methods:
SDC1-expressing (a) or SDC2-expressing (b) cells were plated for 1 h on MatTek 3.5-mm Petri dishes. RITC-labeled polyplexes were then added to cells.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293, -EBNA1