Technical Reference #827

827.

C-Jun Supports Ribosomal Rna Processing And Nucleolar Localization Of Rna Helicase Ddx21 Tim H. Holmstro¨m‡§; Antoine Mialon‡; Marko Kallio‡§; Yvonne Nymalm; Leni Mannermaa‡; Tina Holm; Henrik Johansson; Elizabeth Black**; David Gillespie**‡‡; Tiina A. Salminen; U¨ lo Langel; Benigno C. Valdez§§; and Jukka Westermarck, University of Turku Research Center of Finland University of Stockholm University of Glasgow University of TexasMDAnderson Cancer Center, The Journal of Biological Chemistry, 283(827), (2008)
Link To Paper

Abstract:
The molecular mechanisms by which the AP-1 transcription factor c-Jun exerts its biological functions are not clearlyunderstood. In addition to its well established role in transcriptionalregulation of gene expression several reportshave suggested

Materials & Methods:
For photoswitch and fluorescence recovery after photobleaching (FRAP) studies cells were transfected with PS-CFP2- DDX21 EGFP-DDX21 (15) or EGFP-DDX21 mut and grown on glass-bottom dishes (MatTek).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa, MEF