Technical Reference #815

815.

P2X7 Receptors Mediate Atp Release And Amplification Of Astrocytic Intercellular Ca2 Signaling Sylvia O. Suadicani; Celia F. Brosnan; and Eliana Scemes, Albert Einstein College of Medicine of Yeshiva University, The Journal of Neuroscience, 26(815), (2006)
Link To Paper

Abstract:
Modulation of synaptic transmission and brain microcirculation are new roles ascribed to astrocytes in CNS function. A mechanism bywhich astrocytes modify neuronal activity in the healthy brain depends on fluctuations of cytosolic Ca2levels which regul

Keywords:
ATP release; gap junction blockers; connexin hemichannels; Ca2 waves; glia; astrocyte

Materials & Methods:
Spinal cord astrocytes plated on MatTek dishes were loaded with fura-2 AM (10 M) for 45 min at 37°C and changes in fura-2 fluorescence intensities measured as described above.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Astrocyte