Technical Reference #811

811.

Probing Intranuclear Environments At The Single-Molecule Level David Gru¨ nwald;* Robert M. Martin;y Volker Buschmann;yz David P. Bazett-Jones;§ Heinrich Leonhardt;z Ulrich Kubitscheck;* and M. Cristina Cardoso, Rheinische Friedrich-Wilhelms-University Center for Molecular Medicine Ludwig Maximilians University Munich The Hospital for Sick Children Toronto, Biophysical Journal, 94(811), (2008)
Link To Paper

Abstract:
Genome activity and nuclear metabolism clearly depend on accessibility but it is not known whether and to whatextent nuclear structures limit the mobility and access of individual molecules. We used fluorescently labeled streptavidin with anuclear lo

Materials & Methods:
Mouse C2C12 myoblasts were cultured as described previously (19). For live cell analysis cells were seeded onto either one-well LabTek chambers (Nalge Nunc International Rochester NY) or glass-bottom dishes (MatTek Ashland MA).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
C2C12