Technical Reference #810

810.

Activated Kfras; But Not Hras Or Nras; May Initiate Tumors Of Endodermal Origin Via Stem Cell Expansion Margaret P. Quinlan; Steven E. Quatela; Mark R. Philips; and Jeffrey Settleman, Massachusetts General Hospital Cancer Center, Molecular Cell Biology, 28(810), (2008)
Link To Paper

Abstract:
The three closely related human Ras genes Hras Nras and Kras are all widely expressed engage a common set of downstream effectors and can each exhibit oncogenic activity. However the vast majority of activatingRas mutations in human tumors involv

Materials & Methods:
Undifferentiated F9 cells or F9 cells treated with 200 nM RA for 5 days to induce differentiation were plated at 3 105 cells per plate into 35-mm dishes containing a 15-mm glass coverslip-covered cutout (MatTek).

Microscopic Technique
Fluorescence Microscopy, Immunofluorescence

Cell Type(s)
F9