Technical Reference #809

809.

Real-Time Cross-Correlation Image Analysis Of Early Events In Ige Receptor Signaling. Raibatak Das; Stephanie Hammond; David Holowka and Barbara Baird, Cornell University, Biophysical Journal, 94(809), (2008)
Link To Paper

Abstract:
Signaling in mast cells and basophils is mediated through IgE and its high affinity cell surface receptor FcRI. Crosslinking of the receptors by a cognate multivalent antigen leads to degranulationand release of mediators of the allergic immune respon

Keywords:
RBL mast cells; quantitative microscopy; confocal image processing; GFP-labeled signaling proteins; co-localization analysis; Fc-epsilon-RI.

Materials & Methods:
The cells were plated overnight in 35mm MatTek dishes (MatTek Corporation Ashland MA) at a subconfluent density ( 4 × 105 cells per dish) prior to transfection.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
RBL-2H3