Technical Reference #807

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm dish with a 14-mm microwell (MatTek)

807.

The Nucleolus Exhibits An Osmotically-Regulated Gatekeeping Activity That Controls Nucleolin’S Spatial Dynamics And Functions. Ling Yang ; Jeff M. Reece ; Jaiesoon Cho ;; Carl D. Bortner 4; and Stephen B. Shears, National Institute of Environmental Health Sciences NIH Reecent Technologies Konkuk University, The Journal of Biological Chemistry, 283(807), (2008)
Link To Paper

Abstract:
We demonstrate that physiologically relevant perturbations in the osmotic environment rheostatically regulate a gatekeeping function for thenucleolus that controls nucleolin’s spatial dynamicsand functions. HeLa cells and U2-OS osteosarcomacells wer

Materials & Methods:
One day prior to transfection with MINPP cells were seeded onto 35mm dish with 14mm microwell (MatTek) at a density of 8 x 105 cells/dish

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa